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pRNAT-U6.3/Hygro
| Cat. No. | Size | Price |
|---|---|---|
SD1561-10 ug
| 10 ug | $ 350.00 |
| Full Name | pRNAT-U6.3/Hygro | |
Description: pRNAT-U6.3/Hygro is a GenScript siRNA expression vector. It is designed for mammalian transfection. It has a GFP marker (coral GFP, cGFP) under CMV promoter control can be used to track the transfection efficiency. It uses a enhanced U6 promoter (modified by a CMV enhancer just before the region) for siRNA expression. The siRNA insert can be cloned between the BamH I and Hind III sites.
Storage Buffer and Concentration: Store at -20°C
Download Protocol: tm0169
Forward Sequencing Primer:
DA0011: pRNA-U6 Forward (TACGATACAAGGCTGTTAGAGAG)
Reverse Sequencing Primer:
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)
Detailed Map:
CMV IE: 7-490
Polylinker: 824-893
U6 Promoter: 497-823
CMV Promoter: 932-1519
cGFP: 1536-2255
SV40 Promoter: 2944-3289
Hygromycin: 3312-4337
pUC ori: 5007-5647
Ampicillin: 5795-6655
Vector Sequence and Restriction Enzyme Map
PRODUCTINFO: 20051024032248 (PDF)