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>SD1162 Sequence: Length: 2686
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCA
CAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTG
TTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGC
ACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCC
ATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGT
TTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCG
ACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCT
GTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT
AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCC
GCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGG
AGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCG
GTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACA
GAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAAC
CGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCAC
AAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG
TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC
CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAAAGCTCACGCTGTAGGTAT
CTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAG
CCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGAC
TTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT
GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGT
ATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGC
AAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGA
AAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAAC
GAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATC
CTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT
GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCA
TCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCT
GGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCA
ATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCC
ATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTG
CGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCT
TCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAA
AAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTA
TCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGC
TTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCG
AGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAA
GTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTG
AGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTC
ACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGG
GCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTAT
CAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATA
GGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATC
ATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC
Note: This sequence has been compiled using information from published sequences and other sources as well as from partial sequences obtained by GenScript Corporation. The vector has not been sequenced completely.



NameFreqPositions
AatII12617
AccI1417
AceIII342, 746, 1986
AciI34201, 227, 496, 599, 689, 851, 879, 1006, 1025, 1146, 1391, 1762, 1853, 2090, 2255, 2332, 2441, 2587, 75, 114, 124, 150, 188, 284, 655, 665, 732, 739, 760, 1256, 1400, 2044, 2211, 2540
AclI21924, 2297
AcyI3235, 2235, 2617
AflIII1806
AhaIII31563, 1582, 2274
AluI1643, 62, 307, 400, 445, 470, 565, 629, 747, 973, 1063, 1109, 1366, 1887, 1987, 2050
AlwNI11217
ApaBI1179
ApaLI3177, 1120, 2366
ApoI1450
AsuI6286, 1741, 1820, 1837, 2059, 2675
AvaI1434
AvaII41837, 2059, 1741, 2675
BamHI1429
BbvI1241, 1213, 1216, 1422, 2116, 254, 327, 630, 711, 729, 1148, 1750
BccI31735, 1859, 2146
Bce83I41174, 912, 1451, 2319
BcefI2387, 1292
BcgI12215
BciVI21015, 2542
BetI61012, 1159, 1990, 47, 1779, 2232
BfiI2364, 1744
BglI2245, 1813
BinI10429, 1447, 1544, 2324, 430, 1373, 1459, 1557, 2021, 2342
BsaXI1659
BseMII51081, 1490, 1656, 171, 2196
BseSI3177, 1120, 2366
BseYI11110
BsiI32670, 979, 2363
BsiYI647, 648, 822, 840, 1006, 1285
BsmAI42531, 2684, 51, 1766
Bsp24I21486, 2611
BspHI31526, 2534, 2639
BspLU11I1806
BspMI1413
BsrI111209, 1222, 2170, 365, 391, 606, 1339, 1745, 1863, 1906, 2345
BsrBI3496, 737, 2538
BsrDI21753, 1935
BtsI32092, 593, 2120
Cac8I14136, 284, 304, 308, 401, 405, 409, 597, 626, 735, 821, 858, 1418, 1809
CauII747, 82, 434, 435, 1185, 1881, 2232
CdiI21947, 2351
CfrI3388, 645, 2087
Cfr10I11779
CjeI8182, 833, 1388, 2611, 302, 1309, 1387, 1487
CjePI51339, 2611, 1486, 1606, 2078
CstMI2215, 2052
CviJI4543, 62, 95, 135, 139, 253, 287, 307, 389, 400, 445, 470, 522, 543, 565, 629, 646, 728, 747, 820, 831, 849, 875, 973, 1063, 1109, 1114, 1139, 1218, 1283, 1294, 1337, 1366, 1729, 1741, 1782, 1808, 1812, 1821, 1887, 1977, 1987, 2050, 2088, 2675
CviRI1340, 178, 329, 404, 412, 632, 1121, 1417, 1752, 1842, 2035, 2123, 2367
DdeI6171, 1081, 1490, 1656, 2196, 2622
DpnI15277, 430, 1373, 1448, 1459, 1467, 1545, 1557, 1662, 2003, 2021, 2067, 2325, 2342, 2378
DraII12674
DrdI291, 908
Eam1105I11694
EciI3878, 1024, 1852
Eco31I11766
Eco57I21333, 2381
Eco57MI31333, 1784, 2381
EcoRI1450
EcoRII9354, 545, 833, 954, 967, 47, 434, 1185, 2232
Esp3I22683, 51
FauI5598, 114, 124, 284, 655
FnuDII102, 4, 107, 652, 654, 852, 1433, 1763, 2256, 2588
Fnu4HI1941, 150, 254, 327, 630, 711, 729, 732, 850, 1005, 1148, 1213, 1216, 1422, 1750, 2089, 2116, 2211, 2440
FokI577, 321, 1679, 1860, 2147
GdiII3388, 645, 2087
GsuI11784
HaeI6819, 830, 1282, 388, 645, 2674
HaeII3235, 680, 1050
HaeIII11287, 389, 646, 820, 831, 849, 1283, 1741, 1821, 2088, 2675
HaeIV11694
HgaI4908, 1486, 108, 2236
HgiAI6177, 444, 1120, 2281, 2366, 2589
HgiCI4235, 438, 550, 1647
HgiJII1444
HhaI173, 106, 236, 257, 588, 653, 681, 714, 984, 1051, 1151, 1325, 1434, 1827, 1920, 2257, 2589
Hin4I21694, 1768
HindII1417
HindIII1399
HinfI6420, 641, 706, 781, 1177, 1694
HpaII1348, 82, 435, 524, 1013, 1160, 1186, 1376, 1780, 1814, 1881, 1991, 2233
HphI712, 21, 2173, 2414, 1550, 1777, 2399
Hpy99I5372, 385, 907, 1701, 1964
Hpy178III13369, 423, 610, 1039, 1173, 1271, 1369, 1452, 1526, 1785, 2534, 2639, 2669
Hpy188I1031, 156, 918, 996, 1349, 1483, 1618, 2064, 2075, 2195
KpnI1438
Ksp632I3290, 684, 2488
MaeI4424, 1301, 1554, 1889
MaeII5374, 1509, 1925, 2298, 2618
MaeIII1157, 348, 368, 1163, 1226, 1342, 1625, 1956, 2014, 2167, 2355
MboI15277, 430, 1373, 1448, 1459, 1467, 1545, 1557, 1662, 2003, 2021, 2067, 2325, 2342, 2378
MboII81304, 1456, 291, 685, 1547, 2302, 2380, 2489
McrI5276, 719, 1143, 2066, 2215
MmeI2996, 1180
MnlI1329, 289, 695, 978, 1856, 2062, 427, 662, 921, 1245, 1645, 1726, 2673
MseI13142, 339, 577, 636, 1512, 1564, 1569, 1583, 1636, 1871, 1910, 2275, 2647
MslI31947, 2106, 2465
MstI2256, 1919
MwoI13179, 229, 236, 245, 275, 396, 544, 588, 672, 739, 853, 1425, 1813
NarI1235
NdeI1183
NlaIII1138, 406, 461, 807, 1527, 2018, 2028, 2106, 2142, 2535, 2640
NlaIV11235, 429, 438, 550, 836, 875, 1647, 1741, 1782, 1993, 2583
NspI337, 405, 806
NspBII6112, 306, 628, 1146, 1391, 2332
PasI1433
PfoI146
PleI41177, 420, 706, 1694
PpiI21497, 2340
PpuMI12674
PstI1411
PvuI2276, 2066
PvuII2306, 628
RsaI3168, 439, 2178
SacI1444
SalI1417
SapI1683
ScaI12177
ScrFI1247, 82, 354, 434, 435, 545, 833, 954, 967, 1185, 1881, 2232
SduI5177, 444, 1120, 2281, 2366
SecI5354, 433, 434, 545, 966
SexAI11184
SfaNI8153, 229, 894, 1946, 2386, 78, 208, 2156
SfeI4411, 1071, 1262, 1940
SimI31480, 999, 1768
SmaI1434
SmlI4912, 1174, 1451, 2319
SphI1405
Sse8387I1410
Sse8647I12674
SspI12501
Sth132I2447, 97, 434, 598, 933, 1132, 1141, 1185, 1699, 2232, 2600, 83, 115, 125, 285, 436, 611, 656, 1040, 1478, 1723, 1882, 2245, 2306
StyI1433
TaqI4418, 448, 906, 2350
TatI2167, 2177
TauI7150, 732, 850, 1005, 2089, 2211, 2440
TfiI5641, 781, 420, 706, 1694
TseI1641, 254, 327, 630, 711, 729, 1148, 1213, 1216, 1422, 1750, 2116, 850, 1005, 2089, 2440
Tsp45I457, 368, 1956, 2167
Tsp4CI819, 54, 262, 768, 839, 1309, 1622, 2137
TspDTI4639, 1575, 1677, 1980
TspEI7451, 487, 504, 579, 1567, 1873, 2128
TspGWI22466, 2149
TspRI20392, 593, 702, 1208, 1221, 1492, 1641, 1746, 2093, 2120, 390, 591, 700, 1206, 1219, 1490, 1639, 1744, 2091, 2118
Tth111II31380, 1419, 1413
VspI3576, 635, 1870
XbaI1423
XhoII7429, 1447, 1458, 1544, 1556, 2324, 2341
XmnI12294

Non-Cutting Enzymes:
AarIAflIIAgeIAlfIAloIApaIAscIAsuII
AvrIIBaeIBalIBbvIIBbvCIBclIBglIIBplI
Bpu10IBsaAIBsaBIBsePIBseRIBsgIBsmIBsp1407I
BspMIIBstEIIBstXIBtgZIBtrIClaICspCIDraIII
DsaIEco47IIIEcoNIEcoRVEspIFalIFseIFspAI
HpaIMfeIMluINaeINcoINheINotINruI
OliIPacIPflMIPmaCIPmeIPshAIPsiIPspXI
PsrIRleAIRsrIISacIISanDISauISfiISgfI
SgrAISnaBISpeISplISrfISse232IStuISwaI
TaqIITth111IXcmIXhoIXmaIIII-CeuIPI-SceI