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>SD1176 Sequence: Length: 2710
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCA
CAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTG
TTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGC
ACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCC
ATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGT
TTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCTCGCGAA
TGCATCTAGATATCGGATCCCGGGCCCGTCGACTGCAGAGGCCTGCATGCAAGCTTGGCG
TAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAAC
ATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACA
TTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCAT
TAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCC
TCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCA
AAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCA
AAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGG
CTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCG
ACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTT
CCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTT
TCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGC
TGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTT
GAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATT
AGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGC
TACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAA
AGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTT
TGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCT
ACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTA
TCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAA
AGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATC
TCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT
ACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGC
TCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGT
GGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTA
AGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTG
TCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTT
ACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTC
AGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTT
ACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTC
TGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACC
GCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAA
CTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAAC
TGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAA
AATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTT
TTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAA
TGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCT
GACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGG
CCCTTTCGTC
Note: This sequence has been compiled using information from published sequences and other sources as well as from partial sequences obtained by GenScript Corporation. The vector has not been sequenced completely.



NameFreqPositions
AatII12641
AccI1448
AceIII342, 770, 2010
AciI32201, 227, 520, 623, 713, 875, 903, 1030, 1049, 1170, 1415, 1786, 1877, 2114, 2279, 2356, 2465, 2611, 124, 150, 188, 284, 679, 689, 756, 763, 784, 1280, 1424, 2068, 2235, 2564
AclI21948, 2321
AcyI3235, 2259, 2641
AflIII1830
AhaIII31587, 1606, 2298
AlfI1455
AluI1643, 62, 307, 403, 472, 494, 589, 653, 771, 997, 1087, 1133, 1390, 1911, 2011, 2074
AlwNI11241
ApaI1442
ApaBI1179
ApaLI3177, 1144, 2390
ApoI1396
AsuI8286, 442, 443, 1765, 1844, 1861, 2083, 2699
AvaI1439
AvaII51861, 2083, 443, 1765, 2699
BamHI1435
BbvI1241, 1237, 1240, 1446, 2140, 254, 327, 654, 735, 753, 1172, 1774
BccI31759, 1883, 2170
Bce83I41198, 936, 1475, 2343
BcefI2387, 1316
BcgI12239
BciVI21039, 2566
BetI61036, 1183, 2014, 47, 1803, 2256
BfiI2364, 1768
BglI2245, 1837
BinI10435, 1471, 1568, 2348, 436, 1397, 1483, 1581, 2045, 2366
BsaXI1683
BseMII51105, 1514, 1680, 171, 2220
BseSI4177, 442, 1144, 2390
BseYI11134
BsiI32694, 1003, 2387
BsiYI647, 672, 846, 864, 1030, 1309
BsmI1418
BsmAI42555, 2708, 51, 1790
Bsp24I21510, 2635
BspHI31550, 2558, 2663
BspLU11I1830
BsrI111233, 1246, 2194, 365, 391, 630, 1363, 1769, 1887, 1930, 2369
BsrBI3520, 761, 2562
BsrDI21777, 1959
BtsI32116, 617, 2144
Cac8I14136, 284, 304, 308, 461, 465, 469, 621, 650, 759, 845, 882, 1442, 1833
CauII747, 82, 439, 440, 1209, 1905, 2256
CdiI21971, 2375
CfrI3388, 669, 2111
Cfr10I11803
CjeI8182, 857, 1412, 2635, 302, 1333, 1411, 1511
CjePI51363, 2635, 1510, 1630, 2102
CstMI2215, 2076
CviJI4743, 62, 95, 135, 139, 253, 287, 307, 389, 403, 443, 460, 472, 494, 546, 567, 589, 653, 670, 752, 771, 844, 855, 873, 899, 997, 1087, 1133, 1138, 1163, 1242, 1307, 1318, 1361, 1390, 1753, 1765, 1806, 1832, 1836, 1845, 1911, 2001, 2011, 2074, 2112, 2699
CviRI1540, 178, 329, 421, 454, 464, 468, 656, 1145, 1441, 1776, 1866, 2059, 2147, 2391
DdeI6171, 1105, 1514, 1680, 2220, 2646
DpnI15277, 436, 1397, 1472, 1483, 1491, 1569, 1581, 1686, 2027, 2045, 2091, 2349, 2366, 2402
DraII12698
DrdI291, 932
Eam1105I11718
EciI3902, 1048, 1876
Eco31I11790
Eco57I21357, 2405
Eco57MI31357, 1808, 2405
EcoRI1396
EcoRII9354, 569, 857, 978, 991, 47, 439, 1209, 2256
EcoRV1429
Esp3I22707, 51
FauI5622, 114, 124, 284, 679
FnuDII112, 4, 107, 415, 676, 678, 876, 1457, 1787, 2280, 2612
Fnu4HI1941, 150, 254, 327, 654, 735, 753, 756, 874, 1029, 1172, 1237, 1240, 1446, 1774, 2113, 2140, 2235, 2464
FokI577, 321, 1703, 1884, 2171
GdiII3388, 669, 2111
GsuI11808
HaeI7459, 843, 854, 1306, 388, 669, 2698
HaeII3235, 704, 1074
HaeIII13287, 389, 443, 460, 670, 844, 855, 873, 1307, 1765, 1845, 2112, 2699
HaeIV2429, 1718
HgaI4932, 1510, 108, 2260
HgiAI6177, 402, 1144, 2305, 2390, 2613
HgiCI4235, 408, 574, 1671
HgiJII2402, 442
HhaI173, 106, 236, 257, 612, 677, 705, 738, 1008, 1075, 1175, 1349, 1458, 1851, 1944, 2281, 2613
Hin4I3429, 1718, 1792
HindII1448
HindIII1471
HinfI5665, 730, 805, 1201, 1718
HpaII1348, 82, 440, 548, 1037, 1184, 1210, 1400, 1804, 1838, 1905, 2015, 2257
HphI712, 21, 2197, 2438, 1574, 1801, 2423
Hpy99I6372, 385, 447, 931, 1725, 1988
Hpy178III14369, 414, 425, 634, 1063, 1197, 1295, 1393, 1476, 1550, 1809, 2558, 2663, 2693
Hpy188I1131, 156, 433, 942, 1020, 1373, 1507, 1642, 2088, 2099, 2219
KpnI1408
Ksp632I3290, 708, 2512
MaeI4426, 1325, 1578, 1913
MaeII5374, 1533, 1949, 2322, 2642
MaeIII1157, 348, 368, 1187, 1250, 1366, 1649, 1980, 2038, 2191, 2379
MboI15277, 436, 1397, 1472, 1483, 1491, 1569, 1581, 1686, 2027, 2045, 2091, 2349, 2366, 2402
MboII81328, 1480, 291, 709, 1571, 2326, 2404, 2513
McrI5276, 743, 1167, 2090, 2239
MmeI21020, 1204
MnlI1429, 289, 412, 719, 1002, 1880, 2086, 458, 686, 945, 1269, 1669, 1750, 2697
MseI13142, 339, 601, 660, 1536, 1588, 1593, 1607, 1660, 1895, 1934, 2299, 2671
MslI31971, 2130, 2489
MstI2256, 1943
MwoI13179, 229, 236, 245, 275, 469, 568, 612, 696, 763, 877, 1449, 1837
NarI1235
NdeI1183
NlaIII1138, 466, 485, 831, 1551, 2042, 2052, 2130, 2166, 2559, 2664
NlaIV12235, 408, 435, 442, 574, 860, 899, 1671, 1765, 1806, 2017, 2607
NruI1414
NspI337, 465, 830
NspBII6112, 306, 652, 1170, 1415, 2356
PfoI146
PleI31201, 730, 1718
PpiI21521, 2364
PpuMI12698
PstI1453
PvuI2276, 2090
PvuII2306, 652
RsaI3168, 409, 2202
SacI1402
SalI1448
SapI1707
ScaI12201
ScrFI1247, 82, 354, 439, 440, 569, 857, 978, 991, 1209, 1905, 2256
SduI6177, 402, 442, 1144, 2305, 2390
SecI4354, 439, 569, 990
SexAI11208
SfaNI9153, 229, 422, 918, 1970, 2410, 78, 208, 2180
SfeI4453, 1095, 1286, 1964
SimI31504, 1023, 1792
SmaI1439
SmlI4936, 1198, 1475, 2343
SphI1465
Sse8647I12698
SspI12525
Sth132I2647, 97, 439, 445, 622, 957, 1156, 1165, 1209, 1723, 2256, 2624, 83, 115, 125, 132, 285, 441, 635, 680, 1064, 1502, 1747, 1906, 2269, 2330
StuI1459
TaqI4400, 449, 930, 2374
TatI2167, 2201
TauI7150, 756, 874, 1029, 2113, 2235, 2464
TfiI4665, 805, 730, 1718
TseI1641, 254, 327, 654, 735, 753, 1172, 1237, 1240, 1446, 1774, 2140, 874, 1029, 2113, 2464
Tsp45I457, 368, 1980, 2191
Tsp4CI819, 54, 262, 792, 863, 1333, 1646, 2161
TspDTI4663, 1599, 1701, 2004
TspEI7397, 511, 528, 603, 1591, 1897, 2152
TspGWI22490, 2173
TspRI20392, 617, 726, 1232, 1245, 1516, 1665, 1770, 2117, 2144, 390, 615, 724, 1230, 1243, 1514, 1663, 1768, 2115, 2142
Tth111II31404, 1443, 1437
VspI3600, 659, 1894
XbaI1425
XhoII7435, 1471, 1482, 1568, 1580, 2348, 2365
XmnI12318

Non-Cutting Enzymes:
AarIAflIIAgeIAloIAscIAsuIIAvrIIBaeI
BalIBbvIIBbvCIBclIBglIIBplIBpu10IBsaAI
BsaBIBsePIBseRIBsgIBsp1407IBspMIBspMIIBstEII
BstXIBtgZIBtrIClaICspCIDraIIIDsaIEco47III
EcoNIEspIFalIFseIFspAIHpaIMfeIMluI
NaeINcoINheINotIOliIPacIPasIPflMI
PmaCIPmeIPshAIPsiIPspXIPsrIRleAIRsrII
SacIISanDISauISfiISgfISgrAISnaBISpeI
SplISrfISse232ISse8387IStyISwaITaqIITth111I
XcmIXhoIXmaIIII-CeuIPI-SceI