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>SD1207 Sequence: Length: 4519
GACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATG
CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG
CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGC
TTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTTAATAC
GACTCACTATAGGGAGAGAGAGAGAATTACCCTCACTAAAGGGAGGAGAAGCATGAATTC
CCCAGTGGAAAGACGCGCAGGCAAAACGCACCACGTGACGGAGCGTGACCGCGCGCCGAG
CGCGCGCCAAGGTCGGGCAGGAAGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATA
TACGATACAAGGCTGTTAGAGAGATAATTAGAATTAATTTGACTGTAAACACAAAGATAT
TAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGTTTGCAGTTTTAAAAT
TATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTTGG
GTTTATATATCTTGTGGAAAGGACGCGGGATCCGAGCTCGGTACCAAGCTTAAGTTTAAA
CCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTTGGGAAGACA
ATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCT
GGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGG
TGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTT
TCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGCA
TCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGG
GTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGG
AGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT
CGGTCTATTCTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCTATTGGTTAAAAAATG
AGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTG
TGGAAAGTCCCCAGGCTCCCCAGGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGT
CAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGC
ATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTC
CGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGG
CCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCC
TAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAGCACG
TGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAA
CTAAACCATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGG
AGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTT
CGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGT
GCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTG
GTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGG
CGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTT
CGTGGCCGAGGAGCAGGACTGACACGTGCTACGAGATTTCGATTCCACCGCCGCCTTCTA
TGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGG
GGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTA
CAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAG
TTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAG
CTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCAC
AATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
GAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTC
GTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG
CTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGT
ATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAA
GAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGC
GTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAG
GTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGT
GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGG
AAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCG
CTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGG
TAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCAC
TGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTG
GCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGT
TACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGG
TGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCC
TTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTT
GGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTT
TAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAG
TGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGT
CGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACC
GCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGC
CGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCG
GGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTAC
AGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACG
ATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCC
TCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACT
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTC
AACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAAT
ACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTC
TTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCAC
TCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAA
AACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACT
CATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGG
ATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCG
AAAAGTGCCACCTGACGTC
Note: This sequence has been compiled using information from published sequences and other sources as well as from partial sequences obtained by GenScript Corporation. The vector has not been sequenced completely.



NameFreqPositions
AatII21730, 4514
AccI432, 2322, 2329, 4517
AceIII42643, 3883, 1200, 1904
AciI5261, 349, 661, 859, 886, 1407, 1419, 1428, 1440, 1450, 1461, 1507, 1722, 2088, 2091, 2393, 2496, 2586, 2748, 2776, 2903, 2922, 3043, 3288, 3659, 3750, 3987, 4152, 4229, 4338, 4484, 625, 741, 765, 814, 828, 831, 1742, 1841, 1893, 1996, 2157, 2552, 2562, 2629, 2636, 2657, 3153, 3297, 3941, 4108, 4437
AclI23821, 4194
AcyI51730, 1949, 2132, 4132, 4514
AflII1649
AflIII4228, 1618, 2062, 2703
AhaIII6533, 546, 655, 3460, 3479, 4171
AlfI31288, 1360, 2025
AloI11079
AluI23134, 635, 647, 777, 944, 1201, 1522, 1576, 1586, 1905, 2206, 2339, 2345, 2367, 2462, 2526, 2644, 2870, 3006, 3263, 3784, 3884, 3947
AlwNI275, 3114
ApaBI21298, 1370
ApaLI32033, 3017, 4263
ApoI4127, 295, 1215, 2242
AsuI12217, 386, 1038, 1755, 1810, 1843, 1849, 1958, 3638, 3717, 3734, 3956
AvaI31580, 1766, 1776
AvaII71755, 1810, 1843, 1849, 3734, 3956, 3638
AvrII11559
BalI11689
BamHI1628
BbvI13850, 1984, 2204, 3110, 3113, 3319, 4013, 200, 2527, 2608, 2626, 3045, 3647
BbvII1714
BccI7698, 1041, 1423, 3632, 3756, 4043, 1023
Bce83I43071, 2809, 3348, 4216
BcefI4989, 3189, 1706, 1987
BcgI14112
BciVI3794, 2912, 4439
BclI2665, 1610
BetI81711, 2909, 3056, 3887, 1761, 2017, 3676, 4129
BfiI3301, 1443, 3641
BglI21987, 3710
BglII112
BinI74, 628, 1605, 2161, 3344, 3441, 4221
BplI11503
Bpu10I1179
BsaAI5332, 491, 1030, 1617, 2063
BsaXI31079, 1924, 2556
BseMII839, 2978, 3387, 3553, 109, 760, 1519, 4093
BsePI4351, 360, 362, 1724
BseRI3283, 1540, 2048
BseSI32033, 3017, 4263
BseYI41352, 732, 778, 3007
BsiI22876, 4260
BsiYI16331, 367, 807, 1133, 1278, 1461, 1846, 1872, 1873, 1878, 2152, 2545, 2719, 2737, 2903, 3182
BsmI12272
BsmAI24428, 3663
Bsp24I42132, 3205, 3383, 4508
BspHI23423, 4431
BspLU11I12703
BsrI141116, 3106, 3119, 4067, 74, 302, 1444, 1701, 2503, 3236, 3642, 3760, 3803, 4242
BsrBI5886, 2393, 1740, 2634, 4435
BsrDI23650, 3832
BtgZI2208, 1042
BtrI11820
BtsI43989, 2268, 2490, 4017
Cac8I28120, 317, 351, 360, 362, 693, 724, 728, 870, 884, 927, 1284, 1303, 1356, 1375, 1724, 1960, 1980, 2021, 2031, 2135, 2494, 2523, 2632, 2718, 2755, 3315, 3706
CauII121580, 1581, 1776, 1777, 1813, 1945, 1956, 2017, 2128, 3082, 3778, 4129
CdiI51042, 1635, 3844, 209, 4248
CfrI61689, 1962, 2019, 2043, 2542, 3984
Cfr10I7927, 1761, 1802, 1960, 2021, 2135, 3676
CjeI11698, 1529, 2730, 3285, 4508, 1326, 1911, 2133, 3206, 3284, 3384
CjePI101701, 3236, 4508, 21, 1982, 2132, 3205, 3383, 3503, 3975
CstMI2889, 3949
CviJI7772, 134, 150, 218, 387, 431, 635, 647, 670, 696, 747, 755, 777, 783, 930, 944, 1039, 1181, 1201, 1274, 1347, 1470, 1499, 1505, 1522, 1545, 1557, 1563, 1576, 1586, 1690, 1764, 1876, 1895, 1905, 1959, 1963, 1986, 2020, 2044, 2111, 2138, 2206, 2339, 2345, 2367, 2419, 2440, 2462, 2526, 2543, 2625, 2644, 2717, 2728, 2746, 2772, 2870, 3006, 3011, 3036, 3115, 3180, 3191, 3234, 3263, 3626, 3638, 3679, 3705, 3709, 3718, 3784, 3874, 3884, 3947, 3985
CviRI20165, 415, 527, 1297, 1306, 1369, 1378, 1493, 1569, 2034, 2203, 2271, 2529, 3018, 3314, 3649, 3739, 3932, 4020, 4264
DdeI939, 109, 180, 760, 1519, 2978, 3387, 3553, 4093
DpnI235, 13, 21, 629, 666, 1606, 1611, 1975, 2146, 2162, 3270, 3345, 3356, 3364, 3442, 3454, 3559, 3900, 3918, 3964, 4222, 4239, 4275
DraII1385
DraIII3329, 1030, 2036
DrdI21073, 2805
DsaI31466, 1686, 1988
Eam1105I13591
EciI8764, 1418, 1439, 1449, 1460, 2775, 2921, 3749
Eco31I13663
Eco57I23230, 4278
Eco57MI52171, 3230, 2150, 3681, 4278
EcoRI1295
EcoRII181271, 1280, 1327, 1344, 1846, 1852, 1873, 1878, 1897, 2442, 2730, 2851, 2864, 1580, 1776, 2017, 3082, 4129
FauI9885, 1406, 1427, 2495, 625, 831, 1996, 2157, 2552
FnuDII27119, 207, 229, 314, 350, 352, 361, 363, 624, 803, 827, 847, 1223, 1723, 1725, 1727, 1840, 1892, 2012, 2156, 2549, 2551, 2749, 3330, 3660, 4153, 4485
Fnu4HI2560, 200, 814, 828, 850, 1506, 1893, 1984, 2090, 2204, 2527, 2608, 2626, 2629, 2747, 2902, 3045, 3110, 3113, 3319, 3647, 3986, 4013, 4108, 4337
FokI7737, 2142, 959, 1424, 3576, 3757, 4044
FseI11959
GdiII51962, 2019, 2542, 2043, 3984
GsuI32171, 2150, 3681
HaeI81556, 1689, 1875, 2716, 2727, 3179, 1962, 2542
HaeII4873, 881, 2577, 2947
HaeIII22218, 387, 1039, 1181, 1499, 1505, 1557, 1690, 1876, 1895, 1959, 1963, 2020, 2044, 2543, 2717, 2728, 2746, 3180, 3638, 3718, 3985
HaeIV3622, 1625, 3591
HgaI7312, 622, 1949, 2132, 2805, 3383, 4133
HgiAI10634, 1715, 2033, 3017, 4178, 4263, 804, 874, 882, 4486
HgiCI5640, 991, 1858, 2447, 3544
HgiJII3634, 746, 782
HhaI33118, 198, 315, 351, 353, 360, 362, 364, 804, 817, 826, 848, 874, 882, 1724, 1726, 1839, 1891, 2011, 2155, 2485, 2550, 2578, 2611, 2881, 2948, 3048, 3222, 3331, 3724, 3817, 4154, 4486
Hin4I6622, 629, 1625, 3591, 3665, 1925
HindII532, 1622, 1696, 2329, 4517
HindIII1646
HinfI1335, 173, 241, 401, 1080, 1102, 2081, 2118, 2538, 2603, 2678, 3074, 3591
HpaII26928, 1581, 1712, 1737, 1762, 1777, 1803, 1813, 1862, 1945, 1956, 1961, 2018, 2022, 2128, 2136, 2421, 2910, 3057, 3083, 3273, 3677, 3711, 3778, 3888, 4130
HphI81020, 1672, 4070, 4311, 1719, 3447, 3674, 4296
Hpy99I161729, 1732, 1819, 2328, 2804, 3598, 3861, 4516, 4519, 354, 926, 1735, 1801, 1912, 2089, 2134
Hpy178III147, 17, 206, 1528, 1752, 2507, 2936, 3070, 3168, 3266, 3349, 3423, 3682, 4431
Hpy188I1754, 631, 759, 973, 1518, 1603, 1608, 1922, 2115, 2815, 2893, 3246, 3380, 3515, 3961, 3972, 4092
KpnI1640
Ksp632I32581, 4385, 381
MaeI10687, 787, 879, 1560, 2277, 2337, 2341, 3198, 3451, 3786
MaeII15333, 492, 497, 921, 1031, 1074, 1086, 1618, 1731, 1821, 2064, 3406, 3822, 4195, 4515
MaeIII16335, 345, 494, 573, 843, 855, 1823, 2217, 3060, 3123, 3239, 3522, 3853, 3911, 4064, 4252
MboI235, 13, 21, 629, 666, 1606, 1611, 1975, 2146, 2162, 3270, 3345, 3356, 3364, 3442, 3454, 3559, 3900, 3918, 3964, 4222, 4239, 4275
MboII11170, 381, 714, 3353, 901, 2178, 2582, 3444, 4199, 4277, 4386
McrI71743, 1759, 2019, 2616, 3040, 3963, 4112
MfeI1161
MluI1228
MmeI52893, 3077, 98, 1076, 1921
MnlI30271, 672, 995, 1510, 1516, 1953, 2149, 2334, 2592, 2875, 3753, 3959, 102, 283, 384, 762, 1497, 1503, 1540, 1543, 1555, 1675, 1790, 1925, 2048, 2559, 2818, 3142, 3542, 3623
MseI2569, 131, 234, 454, 534, 547, 650, 656, 822, 1093, 1191, 1208, 1219, 1229, 1630, 2474, 2533, 3409, 3461, 3466, 3480, 3533, 3768, 3807, 4172
MslI33844, 4003, 4362
MstI13816
MwoI26191, 351, 353, 355, 732, 756, 817, 819, 861, 888, 918, 1275, 1298, 1370, 1421, 1500, 1506, 1717, 1987, 2441, 2485, 2569, 2636, 2750, 3322, 3710
NaeI4927, 1960, 2021, 2135
NcoI21466, 1686
NdeI1561
NlaIII19167, 292, 398, 729, 1304, 1376, 1467, 1687, 1966, 2167, 2315, 2358, 2704, 3424, 3915, 3925, 4003, 4039, 4432
NlaIV15628, 640, 970, 991, 1274, 1347, 1858, 2447, 2733, 2772, 3544, 3638, 3679, 3890, 4480
NruI1206
NspI4728, 1303, 1375, 2703
NspBII6661, 776, 2525, 3043, 3288, 4229
PfoI41812, 1845, 1944, 2127
PleI71080, 3074, 35, 241, 1102, 2603, 3591
PmaCI3332, 1617, 2063
PmeI1654
PpiI33394, 1080, 4237
PsiI21158, 2209
PvuI13963
PvuII2776, 2525
RleAI11884
RsaI643, 213, 483, 641, 1909, 4075
SacI1634
SalI332, 2329, 4517
SapI12580
ScaI14074
ScrFI251271, 1280, 1327, 1344, 1580, 1581, 1776, 1777, 1813, 1846, 1852, 1873, 1878, 1897, 1945, 1956, 2017, 2128, 2442, 2730, 2851, 2864, 3082, 3778, 4129
SduI8634, 746, 782, 1715, 2033, 3017, 4178, 4263
SecI16367, 1270, 1279, 1343, 1466, 1501, 1559, 1580, 1686, 1776, 1872, 1878, 1988, 2046, 2442, 2863
SexAI31326, 1851, 3081
SfaNI10958, 1307, 1379, 2235, 2791, 3843, 4283, 57, 738, 4053
SfeI5247, 809, 2968, 3159, 3837
SgrAI11801
SimI63377, 999, 1825, 2015, 2896, 3665
SmaI21580, 1776
SmlI5649, 2809, 3071, 3348, 4216
SphI3728, 1303, 1375
SspI14398
Sth132I3818, 885, 937, 1406, 1427, 1580, 1776, 2017, 2495, 2830, 3029, 3038, 3082, 3596, 4129, 4497, 216, 374, 626, 832, 954, 1582, 1768, 1778, 1814, 1946, 1957, 1997, 2129, 2158, 2508, 2553, 2937, 3375, 3620, 3779, 4142, 4203
StuI11556
StyI7367, 1466, 1559, 1686, 1270, 1279, 1343
TaqI1033, 589, 674, 997, 1746, 2079, 2330, 2803, 4247, 4518
TatI342, 482, 4074
TauI1260, 814, 828, 1506, 1893, 2090, 2629, 2747, 2902, 3986, 4108, 4337
TfiI11173, 401, 2081, 2118, 2538, 2678, 35, 241, 1102, 2603, 3591
TseI20200, 850, 1984, 2204, 2527, 2608, 2626, 3045, 3110, 3113, 3319, 3647, 4013, 60, 1506, 2090, 2747, 2902, 3986, 4337
Tsp45I7335, 345, 494, 855, 1823, 3853, 4064
Tsp4CI10462, 570, 677, 1056, 2326, 2665, 2736, 3206, 3519, 4034
TspDTI9168, 293, 2100, 2536, 3472, 407, 1832, 3574, 3877
TspEI23128, 162, 265, 296, 446, 452, 456, 511, 538, 1216, 1227, 1231, 1314, 1386, 1478, 1628, 2243, 2384, 2401, 2476, 3464, 3770, 4025
TspGWI32, 338, 4363
TspRI24303, 1702, 2268, 2490, 2599, 3105, 3118, 3389, 3538, 3643, 3990, 4017, 301, 1700, 2266, 2488, 2597, 3103, 3116, 3387, 3536, 3641, 3988, 4015
Tth111II43277, 3316, 89, 3310
VspI6453, 1228, 1629, 2473, 2532, 3767
XhoII1012, 628, 1605, 2161, 3344, 3355, 3441, 3453, 4221, 4238
XmaIII12019
XmnI21226, 4191

Non-Cutting Enzymes:
AarIAgeIApaIAscIAsuIIBaeIBbvCIBsaBI
BsgIBsp1407IBspMIBspMIIBstEIIBstXIClaICspCI
Eco47IIIEcoNIEcoRVEspIEsp3IFalIFspAIHpaI
NarINheINotIOliIPacIPasIPflMIPpuMI
PshAIPspXIPsrIPstIRsrIISacIISanDISauI
SfiISgfISnaBISpeISplISrfISse232ISse8387I
Sse8647ISwaITaqIITth111IXbaIXcmIXhoII-CeuI
PI-SceI