PCR and Cloning
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>SD1233 Sequence: Length: 4930
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCG
CGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATT
GACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCA
ATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCC
AAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTA
CATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTAC
CATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGG
ATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACG
GGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGT
ACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTGGATCCCGTCGCTTACC
GATTCAGAATGGTTGATATCCGCCATTCTGAATCGGTAAGCGACGAAGCTTAATAAAGGA
TCTTTTATTTTCATTGGATCTGTGTGTTGGTTTTTTGTGTGCGGCCGCCCTCGACTGTGC
CTTCTAGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCG
GAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGC
GCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCC
GCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCT
CTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAA
AAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGC
CCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACA
CTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTAT
TGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGT
GTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGC
ATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTA
TGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCC
CGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTA
TTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCT
TTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGATGACCGAGTACAAGCCCACGGT
GCGCCTCGCCACCCGCGACGACGTCCCGCGGGCCGTACGCACCCTCGCCGCCGCGTTCGC
CGACTACCCCGCCACGCGCCACACCGTCGACCCGGACCGCCACATCGAGCGGGTCACCGA
GCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGA
CGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTT
CGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACA
GATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGT
CGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGT
GGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCT
CCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACC
GCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGATTCGAATGACCGACCAAGCGACG
CCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTC
GGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAG
TTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGC
ATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAA
CTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAA
TCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATA
CGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTA
ATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAA
TGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCG
CTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAG
GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA
GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTC
CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACA
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCG
ACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCT
CATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT
GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAG
TCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGC
AGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTAC
ACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGA
GTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAG
CAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGG
TCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAA
AGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATA
TATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCG
ATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATA
CGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCG
GCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCT
GCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGT
TCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGC
TCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGA
TCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGT
AAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTC
ATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAA
TAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCA
CATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCA
AGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCT
TCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCC
GCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAA
TATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATT
TAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC
GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG
CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG
CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGC
TTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATT
GATTATTGAC
Note: This sequence has been compiled using information from published sequences and other sources as well as from partial sequences obtained by GenScript Corporation. The vector has not been sequenced completely.



NameFreqPositions
AatII7121, 174, 257, 443, 1640, 2138, 4675
AccI41706, 2486, 2493, 4678
AceIII52807, 4044, 564, 1213, 1739
AciI8358, 86, 98, 112, 279, 507, 620, 705, 872, 899, 1419, 1431, 1440, 1452, 1462, 1473, 1519, 1633, 1646, 1668, 1671, 1689, 1717, 1809, 1874, 1908, 1940, 1959, 2067, 2085, 2092, 2133, 2159, 2179, 2252, 2255, 2557, 2660, 2750, 2912, 2940, 3067, 3086, 3207, 3452, 3820, 3911, 4148, 4313, 4390, 4499, 4645, 4741, 370, 403, 528, 701, 754, 778, 827, 841, 844, 1648, 1729, 1796, 1811, 1817, 1847, 1853, 1893, 2045, 2113, 2321, 2716, 2726, 2793, 2800, 2821, 3317, 3461, 4102, 4269, 4598
AclI23982, 4355
AcyI13121, 174, 257, 443, 1640, 1805, 1936, 1982, 2138, 2217, 2296, 4293, 4675
AflIII22867, 4908
AhaIII33621, 3640, 4332
AlfI21300, 1372
AloI2577, 1092
AluI23565, 647, 790, 957, 1214, 1534, 1588, 1740, 2370, 2503, 2509, 2531, 2626, 2690, 2808, 3034, 3124, 3170, 3427, 3945, 4045, 4108, 4814
AlwNI23278, 4755
ApaBI31310, 1382, 2163
ApaLI33181, 4424, 4712
ApoI31228, 2406, 4807
AsuI1483, 276, 1051, 1650, 1714, 1824, 1871, 1947, 2156, 3799, 3878, 3895, 4117, 4897
AsuII12198
AvaI11592
AvaII101714, 1824, 2156, 3895, 4117, 83, 276, 1871, 1947, 3799
AvrII11571
BalI11971
BamHI1584
BbvI15863, 1912, 2016, 2368, 3274, 3277, 3480, 4174, 1741, 2691, 2772, 2790, 3209, 3808, 4880
BbvII1727
BccI81054, 1435, 2230, 3793, 3917, 4204, 1036, 1921
Bce83I43235, 2973, 3509, 4377
BcefI51002, 1803, 3353, 1652, 2021
BcgI24273, 2016
BciVI3807, 3076, 4600
BetI93073, 3220, 4048, 1711, 1899, 1944, 2033, 3837, 4290
BfiI3294, 1455, 3802
BglI684, 206, 277, 1808, 1939, 3871
BglII14692
BinI16584, 658, 676, 2325, 3505, 3602, 4382, 4684, 585, 2310, 3434, 3517, 3615, 4079, 4400, 4701
BplI11515
Bpu10I14859
BsaAI2338, 1043
BsaXI31092, 1954, 2720
BseMII83142, 3548, 3714, 4719, 773, 1531, 4254, 4789
BsePI12053
BseRI11552
BseSI52063, 2147, 3181, 4424, 4712
BseYI51292, 1364, 745, 791, 3171
BsiI32234, 3040, 4421
BsiYI1443, 820, 1146, 1473, 1873, 1945, 1999, 2032, 2316, 2709, 2883, 2901, 3067, 3346
BsmI12436
BsmAI5429, 1985, 4589, 2078, 3824
Bsp24I4266, 2296, 3544, 4669
BspHI23584, 4592
BspLU11I12867
BspMI12225
BsrI131129, 3270, 3283, 4228, 295, 1456, 2667, 3400, 3803, 3921, 3964, 4403, 4754
BsrBI7899, 2557, 1727, 1891, 2111, 2798, 4596
BsrDI23811, 3993
BstEII11732
BtgZI34888, 349, 1055
BtsI44150, 2432, 2654, 4178
Cac8I2784, 277, 737, 741, 883, 897, 940, 1296, 1315, 1368, 1387, 1648, 1872, 1903, 1957, 2053, 2065, 2181, 2299, 2658, 2687, 2796, 2882, 2919, 3476, 3867, 4800
CauII111592, 1593, 1711, 1899, 2033, 2058, 2186, 2292, 3246, 3939, 4290
CdiI7349, 1055, 1723, 1777, 4005, 4409, 4889
CfrI7702, 1881, 1905, 1971, 2046, 2706, 4145
Cfr10I4940, 1944, 2299, 3837
CjeI111541, 2894, 3449, 4669, 74, 267, 1338, 2297, 3370, 3448, 3545
CjePI11623, 3400, 4669, 266, 601, 1955, 2296, 3544, 3664, 4136, 4701
CspCI1388
CstMI31952, 902, 4110
CviJI8541, 83, 92, 276, 565, 647, 703, 760, 768, 790, 796, 943, 957, 971, 1052, 1194, 1214, 1287, 1359, 1482, 1511, 1517, 1534, 1557, 1569, 1575, 1588, 1611, 1651, 1740, 1770, 1871, 1882, 1902, 1906, 1928, 1947, 1956, 1972, 2047, 2115, 2120, 2184, 2275, 2302, 2370, 2503, 2509, 2531, 2583, 2604, 2626, 2690, 2707, 2789, 2808, 2881, 2892, 2910, 2936, 3034, 3124, 3170, 3175, 3200, 3279, 3344, 3355, 3398, 3427, 3787, 3799, 3840, 3866, 3870, 3879, 3945, 4035, 4045, 4108, 4146, 4752, 4814, 4830, 4898
CviRI201309, 1318, 1381, 1390, 1505, 1581, 1743, 2171, 2367, 2435, 2693, 3182, 3475, 3810, 3900, 4093, 4181, 4425, 4713, 4845
DdeI9773, 1531, 3142, 3548, 3714, 4254, 4719, 4789, 4860
DpnI22585, 659, 677, 1867, 2310, 2326, 3434, 3506, 3517, 3525, 3603, 3615, 3720, 4061, 4079, 4125, 4383, 4400, 4436, 4685, 4693, 4701
DraIII21043, 2164
DrdI21086, 2969
DsaI6360, 580, 1478, 1614, 1646, 1809
Eam1105I13752
EciI10777, 506, 619, 1430, 1451, 1461, 1472, 2939, 3085, 3910
Eco31I22078, 3824
Eco57I23394, 4439
Eco57MI62075, 2335, 3394, 2314, 3842, 4439
EcoRII2189, 282, 1284, 1339, 1356, 1933, 1969, 1999, 2074, 2166, 2606, 2894, 3015, 3028, 1592, 1711, 1899, 2033, 2186, 3246, 4290
EcoRV1615
Esp3I11984
FauI2185, 111, 278, 898, 1418, 1439, 1632, 1645, 1688, 1873, 1958, 2066, 2091, 2178, 2659, 844, 1648, 1729, 1847, 2321, 2716
FnuDII3059, 816, 840, 860, 1236, 1634, 1647, 1672, 1695, 1761, 1763, 1795, 1810, 1875, 1909, 1960, 2054, 2086, 2160, 2320, 2713, 2715, 2913, 3491, 3821, 4314, 4646, 4799, 4887, 4909
Fnu4HI35701, 704, 827, 841, 863, 1518, 1667, 1670, 1741, 1808, 1907, 1912, 1939, 2016, 2045, 2113, 2254, 2368, 2691, 2772, 2790, 2793, 2911, 3066, 3209, 3274, 3277, 3480, 3808, 4147, 4174, 4269, 4498, 4740, 4880
FokI7750, 1596, 2306, 1436, 3737, 3918, 4205
GdiII6702, 2046, 2706, 1881, 1905, 4145
GsuI42075, 2335, 2314, 3842
HaeI91568, 1927, 1971, 2880, 2891, 3343, 1870, 1946, 2706
HaeII7886, 894, 1805, 1936, 2018, 2741, 3111
HaeIII2583, 276, 703, 1052, 1194, 1511, 1517, 1569, 1651, 1871, 1882, 1906, 1928, 1947, 1972, 2047, 2707, 2881, 2892, 2910, 3344, 3799, 3879, 4146, 4898
HaeIV13752
HgaI9517, 2217, 2296, 2969, 3544, 1764, 1839, 1983, 4294
HgiAI122027, 3181, 4339, 4424, 4712, 817, 887, 895, 1955, 2087, 2161, 4647
HgiCI9465, 1004, 1805, 1936, 2062, 2146, 2189, 2611, 3705
HgiJII5759, 795, 970, 1769, 1955
HhaI34817, 830, 839, 861, 887, 895, 1621, 1696, 1762, 1806, 1876, 1910, 1937, 2019, 2053, 2055, 2087, 2161, 2319, 2649, 2714, 2742, 2775, 3045, 3112, 3212, 3386, 3492, 3885, 3978, 4315, 4647, 4798, 4878
Hin4I23752, 3826
HindII41706, 2493, 4678, 4912
HindIII1646
HinfI14410, 601, 630, 1093, 1115, 2196, 2245, 2282, 2702, 2767, 2842, 3238, 3752, 4853
HpaII21941, 1593, 1712, 1832, 1900, 1945, 2034, 2058, 2187, 2292, 2300, 2585, 3074, 3221, 3247, 3437, 3838, 3872, 3939, 4049, 4291
HphI10364, 1033, 4231, 4472, 1734, 2124, 2130, 3608, 3835, 4457
Hpy99I32590, 641, 1636, 1639, 1705, 1765, 1801, 1840, 1978, 2023, 2137, 2140, 2216, 2492, 2968, 3759, 4022, 4677, 4680, 939, 1666, 1669, 1678, 1807, 1830, 1861, 1938, 2020, 2056, 2134, 2253, 2298
Hpy178III16723, 1540, 1821, 2233, 2671, 3100, 3234, 3332, 3430, 3510, 3584, 3843, 4592, 4687, 4697, 4886
Hpy188I15604, 627, 772, 986, 1530, 2279, 2979, 3057, 3410, 3541, 3676, 4122, 4133, 4253, 4734
Ksp632I31751, 2745, 4546
MaeI11724, 800, 892, 1572, 2441, 2501, 2505, 3362, 3612, 3947, 4930
MaeII16122, 134, 175, 258, 339, 444, 934, 1044, 1087, 1099, 1641, 2139, 3567, 3983, 4356, 4676
MaeIII1662, 149, 498, 856, 868, 1733, 2129, 2381, 3224, 3287, 3403, 3683, 4014, 4072, 4225, 4413
MboI22585, 659, 677, 1867, 2310, 2326, 3434, 3506, 3517, 3525, 3603, 3615, 3720, 4061, 4079, 4125, 4383, 4400, 4436, 4685, 4693, 4701
MboII12727, 3365, 3514, 4850, 914, 1752, 2342, 2746, 3605, 4360, 4438, 4547
McrI6702, 2046, 2780, 3204, 4124, 4273
MfeI14841
MluI14908
MmeI43057, 3241, 1089, 4778
MnlI31709, 1008, 1522, 1528, 1624, 1663, 1756, 1930, 2082, 2098, 2313, 2498, 2756, 3039, 3914, 4120, 548, 775, 1509, 1515, 1552, 1555, 1567, 2042, 2144, 2723, 2982, 3306, 3703, 3784, 4782
MseI207, 650, 835, 1106, 1204, 1221, 1232, 1242, 2638, 2697, 3570, 3622, 3627, 3641, 3694, 3929, 3968, 4333, 4749, 4811
MslI4361, 4005, 4164, 4523
MstI13977
MwoI3884, 206, 238, 277, 370, 394, 745, 769, 830, 832, 874, 901, 931, 1310, 1382, 1433, 1512, 1518, 1612, 1670, 1762, 1808, 1853, 1903, 1939, 1948, 2048, 2163, 2172, 2220, 2605, 2649, 2733, 2800, 2914, 3483, 3871, 4871
NaeI2940, 2299
NarI21805, 1936
NcoI2360, 1478
NdeI1233
NlaIII19301, 361, 742, 1316, 1388, 1479, 1879, 2173, 2331, 2479, 2522, 2868, 3585, 4076, 4086, 4164, 4200, 4593, 4847
NlaIV23465, 584, 971, 983, 1004, 1287, 1359, 1805, 1895, 1936, 1954, 1965, 2062, 2146, 2189, 2611, 2897, 2936, 3705, 3799, 3840, 4051, 4641
NotI1701
NruI14886
NspI4741, 1315, 1387, 2867
NspBII7789, 1646, 1809, 2689, 3207, 3452, 4390
PfoI22073, 2291
PleI61093, 3238, 410, 1115, 2767, 3752
PpiI41748, 3555, 1093, 4398
PsiI21171, 2373
PvuI14124
PvuII2789, 2689
RleAI11788
RsaI11218, 243, 298, 331, 382, 539, 1606, 1655, 4236, 4723, 4893
RsrII21713, 1870
SacII21646, 1809
SalI31706, 2493, 4678
SapI12744
ScaI14235
ScrFI2589, 282, 1284, 1339, 1356, 1592, 1593, 1711, 1899, 1933, 1969, 1999, 2033, 2058, 2074, 2166, 2186, 2292, 2606, 2894, 3015, 3028, 3246, 3939, 4290
SduI12759, 795, 970, 1769, 1955, 2027, 2063, 2147, 3181, 4339, 4424, 4712
SecI17360, 580, 1283, 1355, 1478, 1513, 1571, 1592, 1614, 1646, 1809, 1950, 1999, 2032, 2058, 2606, 3027
SexAI31338, 2165, 3245
SfaNI101319, 1391, 2399, 2955, 4004, 4444, 367, 751, 4214, 4737
SfeI4822, 3132, 3323, 3998
SimI1126, 1731, 1791, 2008, 3538, 107, 1012, 1709, 2176, 3060, 3826
SmaI11592
SmlI42973, 3235, 3509, 4377
SnaBI1338
SpeI14929
SphI3741, 1315, 1387
SplI11654
SspI14559
Sth132I5585, 111, 278, 588, 898, 950, 1418, 1439, 1592, 1632, 1645, 1688, 1711, 1873, 1899, 1958, 1991, 2033, 2066, 2091, 2150, 2178, 2186, 2659, 2994, 3193, 3202, 3246, 3757, 4290, 4658, 4698, 24, 416, 479, 845, 967, 1594, 1649, 1730, 1768, 1848, 2059, 2293, 2322, 2672, 2717, 3101, 3536, 3781, 3940, 4303, 4364, 4688, 4896
StuI21568, 1927
StyI8360, 1478, 1571, 1950, 1283, 1355, 1614, 2032
TaqI13711, 1010, 1707, 1725, 1773, 1842, 2142, 2199, 2243, 2494, 2967, 4408, 4679
TatI7217, 297, 330, 381, 1605, 4235, 4722
TauI20701, 704, 827, 841, 1518, 1667, 1670, 1808, 1907, 1939, 2045, 2113, 2254, 2793, 2911, 3066, 4147, 4269, 4498, 4740
TfiI12601, 630, 2196, 2245, 2282, 2702, 2842, 4853, 410, 1115, 2767, 3752
TseI28863, 1741, 1912, 2016, 2368, 2691, 2772, 2790, 3209, 3274, 3277, 3480, 3808, 4174, 4880, 704, 1518, 1667, 1670, 1808, 1907, 1939, 2254, 2911, 3066, 4147, 4498, 4740
Tsp45I5868, 1733, 2129, 4014, 4225
Tsp4CI1774, 196, 267, 541, 579, 714, 1069, 1616, 1703, 1976, 2126, 2490, 2829, 2900, 3370, 3680, 4195
TspDTI82264, 2700, 3633, 4848, 36, 670, 3735, 4038
TspEI1619, 1229, 1240, 1244, 1326, 1398, 1490, 2407, 2548, 2565, 2640, 3625, 3931, 4186, 4808, 4842
TspGWI34524, 4682, 4207
TspRI202432, 2654, 2763, 3269, 3282, 3550, 3699, 3804, 4151, 4178, 2430, 2652, 2761, 3267, 3280, 3548, 3697, 3802, 4149, 4176
Tth111I11637
Tth111II43441, 3477, 3471, 4769
VspI56, 1241, 2637, 2696, 3928
XbaI1723
XhoII11584, 658, 676, 2325, 3505, 3516, 3602, 3614, 4382, 4399, 4692
XmaIII2702, 2046
XmnI21239, 4352

Non-Cutting Enzymes:
AarIAflIIAgeIApaIAscIBaeIBbvCIBclI
BsaBIBsgIBsp1407IBspMIIBstXIBtrIClaIDraII
Eco47IIIEcoNIEcoRIEspIFalIFseIFspAIHpaI
KpnINheIOliIPacIPasIPflMIPmaCIPmeI
PpuMIPshAIPspXIPsrIPstISacISanDISauI
SfiISgfISgrAISrfISse232ISse8387ISse8647ISwaI
TaqIIXcmIXhoII-CeuIPI-SceI