PCR and Cloning
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>SD1801 Sequence: Length: 5132
AATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTTGGGTTTATATAT
CTTGTGGAAAGGACGCGGGATCCCGTCGCTTACCGATTCAGAATGGTTGATATCCGCCAT
TCTGAATCGGTAAGCGATTTTTTCCAACGAAGCTTAAGTTTAAACCGCTGATCAGCCTCG
ACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACC
CTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGT
CTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGAT
TGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAA
AGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCG
GCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT
CCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTA
AATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAA
CTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCT
TTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTC
AACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGG
TTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTC
AGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATC
TCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGC
AAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGC
CCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTT
ATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTT
TTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTG
ATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTT
CTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCT
GCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGA
CCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGG
CCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACT
GGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCG
AGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCT
GCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCG
GTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGT
TCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATG
CCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCC
GGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAG
AGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATT
CGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTT
CGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGC
CTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCA
GCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAA
TGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCA
TTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGAC
CTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCC
GCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTA
ATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAA
CCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT
TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCG
AGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGC
AGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT
GCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAG
TCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC
CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCC
TTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGT
CGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT
ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGC
AGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA
GTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAA
GCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGG
TAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA
TCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT
TTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAG
TTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT
CAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCC
CGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGAT
ACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAG
GGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTG
CCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGC
TACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCA
ACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGG
TCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGC
ACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTA
CTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTC
AATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACG
TTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACC
CACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGC
AAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAAT
ACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG
CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCC
CCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACT
CTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTG
TTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACC
GACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGG
GCCAGATATACGCGTTTAATACGACTCACTATAGGGAGAGAGAGAGAATTACCCTCACTA
AAGGGAGGAGAAGCATGAATTCCCCAGTGGAAAGACGCGCAGGCAAAACGCACCACGTGA
CGGAGCGTGACCGCGCGCCGAGCGCGCGCCAAGGTCGGGCAGGAAGAGGGCCTATTTCCC
ATGATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTAGAATTAAT
TTGACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTT
GGGTAGTTTGCAGTTTTAAAATTATGTTTTAA
Note: This sequence has been compiled using information from published sequences and other sources as well as from partial sequences obtained by GenScript Corporation. The vector has not been sequenced completely.



NameFreqPositions
AatII14577
AccI32388, 2395, 4580
AceIII32709, 3946, 850
AciI61114, 165, 509, 536, 1056, 1068, 1077, 1089, 1099, 1110, 1156, 1327, 1390, 1892, 1937, 2003, 2029, 2154, 2157, 2459, 2562, 2652, 2814, 2842, 2969, 2988, 3109, 3354, 3722, 3813, 4050, 4215, 4292, 4401, 4547, 4643, 4931, 75, 391, 415, 464, 478, 481, 1484, 1548, 1649, 1652, 1932, 1987, 2085, 2223, 2618, 2628, 2695, 2702, 2723, 3219, 3363, 4004, 4171, 4500
AclI23884, 4257
AcyI51417, 2119, 2198, 4195, 4577
AflII1153
AflIII22769, 4810
AhaIII6159, 3523, 3542, 4234, 5115, 5128
AlfI2937, 1009
AloI1729
AluI24151, 427, 594, 851, 1171, 1225, 1235, 1523, 1981, 2272, 2405, 2411, 2433, 2528, 2592, 2710, 2936, 3026, 3072, 3329, 3847, 3947, 4010, 4716
AlwNI23180, 4657
ApaBI2947, 1019
ApaLI33083, 4326, 4614
ApoI4865, 2308, 4709, 4877
AsuI8688, 1934, 3701, 3780, 3797, 4019, 4799, 4968
AsuII12099
AvaI11229
AvaII41934, 3797, 4019, 3701
AvrII11208
BalI11498
BamHI178
BbvI18500, 1479, 1521, 2042, 2270, 3176, 3179, 3382, 4076, 1378, 1562, 1655, 2593, 2674, 2692, 3111, 3710, 4782
BbvII1364
BccI11202, 691, 1072, 1630, 2132, 3695, 3819, 4106, 673, 1302, 1730
Bce83I53137, 1809, 2875, 3411, 4279
BcefI4639, 1828, 3255, 1392
BcgI14175
BciVI4444, 1626, 2978, 4502
BclI2169, 1259
BetI61449, 2975, 3122, 3950, 3739, 4192
BfiI41357, 1092, 3704, 4883
BglI13773
BglII14594
BinI1978, 1254, 1278, 1589, 1835, 2227, 3407, 3504, 4284, 4586, 79, 1668, 2212, 3336, 3419, 3517, 3981, 4302, 4603
BplI21152, 2083
Bpu10I14761
BsaAI4680, 1720, 4914, 5073
BsaBI11273
BsaXI2729, 2622
BseMII103044, 3450, 3616, 4621, 301, 410, 1168, 2081, 4156, 4691
BsePI41815, 4933, 4942, 4944
BseRI21189, 4865
BseSI51360, 1453, 3083, 4326, 4614
BseYI6929, 1001, 382, 428, 1922, 3073
BsiI42136, 2010, 2942, 4323
BsiYI13457, 783, 1110, 1393, 1937, 2218, 2611, 2785, 2803, 2969, 3248, 4913, 4949
BsmI12338
BsmAI34491, 1266, 3726
Bsp24I32198, 3446, 4571
BspHI23486, 4494
BspLU11I12769
BspMI31677, 2127, 1314
BsrI15766, 1357, 1558, 3172, 3185, 4130, 1093, 2569, 3302, 3705, 3823, 3866, 4305, 4656, 4884
BsrBI6536, 2029, 2459, 2083, 2700, 4498
BsrDI31644, 3713, 3895
BtgZI61855, 4790, 290, 692, 1704, 2048
BtsI44052, 2334, 2556, 4080
Cac8I33197, 374, 378, 520, 534, 577, 933, 952, 1005, 1024, 1310, 1496, 1715, 1781, 1787, 1815, 1819, 1860, 1864, 1918, 2201, 2560, 2589, 2698, 2784, 2821, 3378, 3769, 4702, 4899, 4933, 4942, 4944
CauII81229, 1230, 1421, 1581, 2194, 3148, 3841, 4192
CdiI10290, 692, 1704, 1709, 1907, 2048, 3907, 1856, 4311, 4791
CfrI61324, 1498, 1889, 1916, 2608, 4047
Cfr10I5577, 1737, 1918, 2201, 3739
CjeI10202, 1178, 2796, 3351, 4571, 975, 2199, 3272, 3350, 3447
CjePI9117, 3302, 4571, 95, 2198, 3446, 3566, 4038, 4603
CstMI2539, 4012
CviJI91151, 174, 200, 397, 405, 427, 433, 580, 594, 608, 689, 831, 851, 924, 996, 1119, 1148, 1154, 1171, 1194, 1206, 1212, 1225, 1235, 1325, 1342, 1350, 1377, 1402, 1486, 1495, 1499, 1523, 1561, 1637, 1654, 1673, 1736, 1780, 1790, 1807, 1890, 1917, 1921, 1958, 1981, 1997, 2177, 2204, 2272, 2405, 2411, 2433, 2485, 2506, 2528, 2592, 2609, 2691, 2710, 2783, 2794, 2812, 2838, 2936, 3026, 3072, 3077, 3102, 3181, 3246, 3257, 3300, 3329, 3689, 3701, 3742, 3768, 3772, 3781, 3847, 3937, 3947, 4010, 4048, 4654, 4716, 4732, 4800, 4969, 5013
CviRI25288, 946, 955, 1018, 1027, 1142, 1218, 1309, 1468, 1643, 1657, 2269, 2337, 2595, 3084, 3377, 3712, 3802, 3995, 4083, 4327, 4615, 4747, 4997, 5109
DdeI11301, 410, 1168, 2081, 3044, 3450, 3616, 4156, 4621, 4691, 4762
DpnI2879, 170, 1255, 1260, 1279, 1590, 1668, 1749, 1758, 1836, 2212, 2228, 3336, 3408, 3419, 3427, 3505, 3517, 3622, 3963, 3981, 4027, 4285, 4302, 4338, 4587, 4595, 4603
DraII14967
DraIII2680, 4911
DrdI3723, 1439, 2871
DsaI21115, 1850
Eam1105I13654
EciI10414, 1931, 113, 1067, 1088, 1098, 1109, 2841, 2987, 3812
Eco31I13726
Eco57I41543, 1975, 3296, 4341
Eco57MI71543, 1975, 2237, 3296, 2216, 3744, 4341
EcoRI14877
EcoRII13240, 921, 976, 993, 1804, 2508, 2796, 2917, 2930, 1229, 1421, 3148, 4192
EcoRV1109
FalI11735
FauI10535, 1055, 1076, 2561, 75, 481, 1548, 2085, 2223, 2618
FnuDII2474, 453, 477, 497, 873, 1483, 1784, 1816, 2222, 2615, 2617, 2815, 3393, 3723, 4216, 4548, 4701, 4789, 4811, 4896, 4932, 4934, 4943, 4945
Fnu4HI37464, 478, 500, 1155, 1326, 1378, 1389, 1479, 1484, 1521, 1562, 1649, 1652, 1655, 1891, 1987, 2028, 2042, 2156, 2270, 2593, 2674, 2692, 2695, 2813, 2968, 3111, 3176, 3179, 3382, 3710, 4049, 4076, 4171, 4400, 4642, 4782
FokI9387, 1272, 1729, 1754, 2208, 1073, 3639, 3820, 4107
GdiII51324, 2608, 1889, 1916, 4047
GsuI32237, 2216, 3744
HaeI61205, 1498, 2782, 2793, 3245, 2608
HaeII5523, 531, 1417, 2643, 3013
HaeIII19689, 831, 1148, 1154, 1206, 1325, 1499, 1890, 1917, 2609, 2783, 2794, 2812, 3246, 3701, 3781, 4048, 4800, 4969
HaeIV372, 1590, 3654
HgaI772, 2119, 2198, 2871, 3446, 4894, 4196
HgiAI111527, 1717, 3083, 4241, 4326, 4614, 454, 524, 532, 1418, 4549
HgiCI6247, 641, 1417, 1452, 2513, 3607
HgiJII4396, 432, 607, 1779
HhaI36454, 467, 476, 498, 524, 532, 1410, 1418, 1482, 1519, 1785, 1815, 1817, 2045, 2221, 2551, 2616, 2644, 2677, 2947, 3014, 3114, 3288, 3394, 3787, 3880, 4217, 4549, 4700, 4780, 4897, 4933, 4935, 4942, 4944, 4946
Hin4I672, 1590, 3654, 3728, 303, 1620
HindII22395, 4580
HindIII1150
HinfI1795, 124, 730, 752, 1903, 2037, 2089, 2147, 2184, 2604, 2669, 2744, 3140, 3654, 4755, 4823, 4983
HpaII22578, 1230, 1323, 1400, 1422, 1450, 1581, 1671, 1738, 1919, 2194, 2202, 2487, 2976, 3123, 3149, 3339, 3740, 3774, 3841, 3951, 4193
HphI7670, 4133, 4374, 1604, 3510, 3737, 4359
Hpy99I1884, 1504, 1532, 1826, 1841, 2118, 2394, 2870, 3661, 3924, 4579, 4582, 576, 1391, 2027, 2155, 2200, 4936
Hpy178III231177, 1262, 1435, 1751, 1760, 1843, 1899, 1943, 2033, 2066, 2135, 2573, 3002, 3136, 3234, 3332, 3412, 3486, 3745, 4494, 4589, 4599, 4788
Hpy188I2198, 121, 300, 409, 623, 1167, 1252, 1257, 1383, 1727, 2080, 2181, 2881, 2959, 3312, 3443, 3578, 4024, 4035, 4155, 4636
Ksp632I52647, 4448, 1767, 1977, 4963
MaeI10191, 437, 529, 1209, 2343, 2403, 2407, 3264, 3514, 3849
MaeII13571, 681, 724, 736, 1534, 1721, 3469, 3885, 4258, 4578, 4915, 5074, 5079
MaeIII1723, 493, 505, 1539, 1845, 2283, 3126, 3189, 3305, 3585, 3916, 3974, 4127, 4315, 4917, 4927, 5076
MboI2879, 170, 1255, 1260, 1279, 1590, 1668, 1749, 1758, 1836, 2212, 2228, 3336, 3408, 3419, 3427, 3505, 3517, 3622, 3963, 3981, 4027, 4285, 4302, 4338, 4587, 4595, 4603
MboII15364, 1767, 1977, 3267, 3416, 4752, 4963, 551, 2077, 2244, 2648, 3507, 4262, 4340, 4449
McrI51324, 2682, 3106, 4026, 4175
MfeI14743
MluI14810
MmeI5143, 2959, 3143, 726, 4680
MnlI33176, 218, 645, 1159, 1165, 2009, 2215, 2400, 2658, 2941, 3816, 4022, 4853, 280, 355, 412, 1146, 1152, 1189, 1192, 1204, 1276, 1340, 1476, 1833, 2625, 2884, 3208, 3605, 3686, 4684, 4865, 4966
MseI24154, 160, 472, 743, 841, 858, 869, 879, 2540, 2599, 3472, 3524, 3529, 3543, 3596, 3831, 3870, 4235, 4651, 4713, 4816, 5036, 5116, 5129
MslI41851, 3907, 4066, 4425
MstI21518, 3879
MwoI32382, 406, 467, 469, 511, 538, 568, 947, 1019, 1070, 1149, 1155, 1403, 1487, 1510, 1649, 1655, 1772, 1808, 1855, 2122, 2507, 2551, 2635, 2702, 2816, 3385, 3773, 4773, 4933, 4935, 4937
NaeI3577, 1918, 2201
NarI11417
NcoI21115, 1850
NdeI111
NlaIII22379, 953, 1025, 1116, 1289, 1634, 1820, 1851, 1877, 2233, 2381, 2424, 2770, 3487, 3978, 3988, 4066, 4102, 4495, 4749, 4874, 4980
NlaIV1778, 247, 608, 620, 641, 924, 996, 1417, 1452, 2513, 2799, 2838, 3607, 3701, 3742, 3953, 4543
NruI14788
NspI5378, 952, 1024, 1819, 2769
NspBII7165, 426, 1522, 2591, 3109, 3354, 4292
PfoI12193
PleI7730, 3140, 752, 2089, 2669, 3654, 4823
PmaCI14914
PmeI1158
PpiI33457, 730, 4300
PsiI2808, 2275
PstI11467
PvuI14026
PvuII3426, 1522, 2591
RsaI51723, 4138, 4625, 4795, 5065
RsrII11933
SalI22395, 4580
SapI32646, 1767, 1977
ScaI14137
ScrFI17240, 921, 976, 993, 1229, 1230, 1421, 1581, 1804, 2194, 2508, 2796, 2917, 2930, 3148, 3841, 4192
SduI12396, 432, 607, 1360, 1453, 1527, 1717, 1779, 3083, 4241, 4326, 4614
SecI12239, 920, 992, 1115, 1150, 1208, 1229, 1581, 1850, 2508, 2929, 4949
SexAI2975, 3147
SfaNI16289, 956, 1028, 1708, 1772, 2047, 2301, 2857, 3906, 4346, 388, 1386, 1641, 1857, 4116, 4639
SfeI6459, 1467, 3034, 3225, 3900, 4829
SimI63440, 237, 649, 1847, 2962, 3728
SmaI11229
SmlI6153, 1809, 2875, 3137, 3411, 4279
SphI4378, 952, 1024, 1819
SspI14461
Sth132I4082, 223, 535, 587, 1055, 1076, 1229, 1421, 1824, 1963, 2034, 2561, 2896, 3095, 3104, 3148, 3659, 4192, 4560, 4600, 482, 604, 1231, 1507, 1549, 1582, 2086, 2195, 2224, 2574, 2619, 3003, 3438, 3683, 3842, 4205, 4266, 4590, 4798, 4956
StuI11205
StyI61115, 1208, 1850, 4949, 920, 992
TaqI1439, 178, 647, 1531, 1687, 1711, 1747, 1909, 2100, 2145, 2396, 2869, 4310, 4581
TatI34137, 4624, 5064
TauI19464, 478, 1155, 1326, 1389, 1484, 1649, 1652, 1891, 1987, 2028, 2156, 2695, 2813, 2968, 4049, 4171, 4400, 4642
TfiI1595, 124, 1903, 2037, 2147, 2184, 2604, 2744, 4755, 4983, 752, 2089, 2669, 3654, 4823
TseI29500, 1378, 1479, 1521, 1562, 1655, 2042, 2270, 2593, 2674, 2692, 3111, 3176, 3179, 3382, 3710, 4076, 4782, 1155, 1326, 1389, 1891, 2028, 2156, 2813, 2968, 4049, 4400, 4642
Tsp45I8505, 1539, 1845, 3916, 4127, 4917, 4927, 5076
Tsp4CI1420, 181, 259, 706, 1796, 1912, 2020, 2392, 2731, 2802, 3272, 3582, 4097, 5044
TspDTI101462, 2166, 2602, 3535, 4750, 4875, 1905, 3637, 3940, 4989
TspEI23285, 866, 877, 881, 963, 1035, 1127, 2309, 2450, 2467, 2542, 3527, 3833, 4088, 4710, 4744, 4847, 4878, 5028, 5034, 5038, 5093, 5120
TspGWI44426, 4584, 4920, 4109
TspRI26258, 1541, 2334, 2556, 2665, 3171, 3184, 3452, 3601, 3706, 4053, 4080, 4885, 256, 1539, 2332, 2554, 2663, 3169, 3182, 3450, 3599, 3704, 4051, 4078, 4883
Tth111I11533
Tth111II63343, 3379, 210, 1863, 3373, 4671
VspI5878, 2539, 2598, 3830, 5035
XhoII1278, 1254, 1589, 1835, 2227, 3407, 3418, 3504, 3516, 4284, 4301, 4594
XmaIII11324
XmnI2876, 4254

Non-Cutting Enzymes:
AarIAgeIApaIAscIBaeIBbvCIBsgIBsp1407I
BspMIIBstEIIBstXIBtrIClaICspCIEco47IIIEcoNI
EspIEsp3IFseIFspAIHpaIKpnINheINotI
OliIPacIPasIPflMIPpuMIPshAIPspXIPsrI
RleAISacISacIISanDISauISfiISgfISgrAI
SnaBISpeISplISrfISse232ISse8387ISse8647ISwaI
TaqIIXbaIXcmIXhoII-CeuIPI-SceI