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HRP-Protein G

Cat. No.	Size	Price
M00090-1 mg	1 mg	$ 75.00
Full Name	HRP-Protein G

Description:

Protein G, a cell surface protein of Group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A. It contains 3 IgG binding domains and is engineered to delete albumin-binding region. HRP has been covalently coupled to Protein G to produce the HRP-Protein G, which can be used in detection systems such as immunoblotting, ELISA, immunoperoxidase electron microscopy and immunohistochemistry.

Concentration:

1 mg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal.

Storage:

HRP-Protein G should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freezing and thawing cycles.

Reconstitution:

Reconstitute the lyophilized power with 1.0 ml of deionized water to a final HRP-Protein G concentration of 1 mg/ml.

Application:

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including primary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Dot blot: 1:2,000-1:10,000
Western blot: 1:2,000-1:10,000
ELISA: 1:10,000-1:50,000
Immunohistology: 1:1,000-1:10,000

PRODUCTINFO:  20060920233835  (PDF)
MSDS:  20080916031204  (PDF)

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