You are here:  Products » Proteins, Expression, Isolation and Analysis » Protein Detection and Immunoassay  » Reagents for Protein Analysis »  M00096

Biotin-Protein G

Cat. No.	Size	Price
M00096-1 mg	1 mg	$ 98.00
Full Name	Biotin-Protein G

Description:

Protein G, a cell surface protein from Group G streptococci, binds strongly and specifically to the Fc region of immunoglobulin molecules, particularly IgG. It contains three high-affinity binding sites that are capable of interacting with the Fc region of IgG in humans, rabbits, mice, cows, goats, sheep, and other species. Biotin-Protein G, which is produced by covalently linking biotin to protein G, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.

Concentration:

1 mg/ml in PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide.

Storage:

Store at 4°C for short periods of time, one month or shorter. Aliquot and store at -20°C for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use.

Application:

Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration.
Dot blot: 1:2,000-1:50,000
Western blot: 1:2,000-1:50,000
ELISA: 1:50,000-1:200,000
Other: user-optimized

DATASHEET:  20070208032719  (PDF)

1	Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology.
2	PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
3	Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
4	Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.