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Biotin-Protein L
| Cat. No. | Size | Price |
|---|---|---|
M00097-1 mg
| 1 mg | $ 98.00 |
| Full Name | Biotin-Protein L | |
Description:
Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.Concentration:
1 mg/ml in PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide.Storage:
Store at 4°C for short periods of time, one month or shorter. Aliquot and store at -20°C for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use.Application:
Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration.Dot blot: 1:2,000-1:20,000
Western blot: 1:2,000-1:20,000
ELISA: 1:20,000-1:100,000
Other: user-optimized
PRODUCTINFO: 20070208033901 (PDF)