You are here:  Biology CRO for Drug Discovery » Bio-Assay Center » Cell Lines and Membranes » GPCR & Ion Channel Cell Lines »  M00131

293/H1 Stable Cell Line

Cat. No.	Size	Price
M00131-2 vials	2 vials
Full Name	293/H1 Stable Cell Line

Species:

Human

Description:

The H1 histamine receptor is expressed primarily in the lungs, vasculature, and brain. The H1 mediates the contraction of smooth muscles, neurotransmission in the central nervous system, the release of catecholamine from adrenal medulla, and increases in capillary permeability due to contraction of terminal venules. H1's role in inflammatory responses makes its antagonist suitable for treating allergies.

Storage:

Liquid nitrogen immediately upon delivery

Application:

Functional assays for H1 receptor

Application Examples:

Intracellular calcium response from 293 cells stably expressing human H1 histamine receptors and from untransfected control cells. Cells were loaded with Calcium-4 then stimulated with the indicated concentrations of histamine. Calcium responses were recorded on a FlexStation plate reader. Data represent the average +/- standard deviation of triplicate determinations.

PROTOCOL:  20080905022527  (PDF)
MSDS:  20090813055625  (PDF)

EXAMPLE:

Zoom

1	Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology.
2	PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
3	Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
4	Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.