WI38
| Cat. No. | Size | Price |
|---|
M0015-100 ug
| 100 ug | $ 71.50 |
|---|
| Full Name | WI38 |
|---|
Species:
Human
Tissue:
lung
Description:
WI-38 cell (human lung fibroblasts) lysate is prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, and 5 µg/ml of leupeptin). Cell debris are removed by centrifugation. Protein concentration is determined via Bio-Rad protein assay. The cell lysate is boiled for five minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, and 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Concentration:
The product is supplied at 100 μg/100 μl. whole cell lysate, in 1 x SDS sample buffer containing 5% b-mercaptoethanol.
Note:
Cell line: WI-38 (Human lung fibroblast, normal)
Growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, and 10% FBS
Storage:
Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity:
Whole cell lysate
Application:
WI-38 cell lysate is delivered ready-to-load. It is ideal for SDS-PAGE and Western blotting. We recommend 10 - 20 µg per lane isrecommended for mini-gels.
