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T-47D

Cat. No.	Size	Price
M0023-100 ug	100 ug	$ 72.00
Full Name	T-47D

Species:

Human

Tissue:

Other

Description:

This product consists of lysed human breast carcinoma cells. T47D cells carry receptors for a variety of steroids and calcitonins. They are responsive to estrogen and mutant tumor suppressor protein p53. Under normal culturing conditions, they express progesterone receptor constitutively. They are able to lose the estrogen receptor (ER) during long-term estrogen deprivation in vitro. These cells are therefore used as a model for studies of drug resistance to tamoxifen in patients with mutant p53 breast tumors. 100 μg/100 μl.

Concentration:

Concentration: 100 μg/100 μl. Preservative: None Constituents: 20 % glycerol, 20 mM HEPES, 100 mM potassium chloride, 1mM Magnesium Chloride, 0.5 mM PMSF, and 0.5 mM DTT. pH 7.9

Note:

These extracts have been quality control tested by Western blot and the electrophoretic mobility shift assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Storage:

Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Purity:

Whole cell lysate

Application:

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

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