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293

Cat. No.	Size	Price
M0032-100 ug	100 ug	$ 71.50
Full Name	293

Species:

Human

Tissue:

Genito-urinary

Description:

293 whole cell lysate.

Concentration:

Concentration: 100 μg/100 μl
Storage buffer: Whole lysate in 1 x SDS sample buffer containing 5% b-mercaptoethanol

Volume:

20 μg per lane.

Storage:

The lysate may be store at -20˚C for three months or at –70˚C for one year.

Background:

293 cell lysate is prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris is removed by centrifugation. Protein concentration was determined via Bio-Rad protein assay. The cell lysate is boiled for five minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, and 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

EXAMPLE:

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