T-24
| Cat. No. | Size | Price |
|---|
M0051-100 ug
| 100 ug | $ 71.50 |
|---|
| Full Name | T-24 |
|---|
Species:
Human
Tissue:
Genito-Urinary
Description:
Human urinary carcinoma. T24 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Concentration:
100 μg/100 μl. Whole lysate in 1 X SDS 5% b-mercaptoethanol.
Note:
Cell line: T24 (Transitional-cell human bladder carcinoma)
Growth media: DMEM & 10% FBS (Fetal bovine serum).
Storage:
Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity:
Whole Cell Lysate.
Application:
T24 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane isrecommended for mini gel.
