Proteins, Expression, Isolation and Analysis
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T-24


Cat. No.SizePrice
M0051-100 ug
 		100 ug $ 72.00
Full NameT-24

Species:
Human
Tissue:
Genito-Urinary
Description:
Human urinary carcinoma. T24 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Concentration:
100 μg/100 μl. Whole lysate in 1 X SDS 5% b-mercaptoethanol.

Note:
Cell line: T24 (Transitional-cell human bladder carcinoma)
Growth media: DMEM & 10% FBS (Fetal bovine serum).

Storage:
Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Purity:
Whole Cell Lysate.

Application:
T24 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane isrecommended for mini gel.



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