Proteins, Expression, Isolation and Analysis
You are here:  Products » Proteins, Expression, Isolation and Analysis » Protein Detection and Immunoassay  » Cell Lysates »  M0052   

B16


Cat. No.SizePrice
M0052-100 ug
 		100 ug $ 72.00
Full NameB16

Species:
Mouse
Tissue:
Other
Description:
Murine melanoma; 100 μg/100 μl.
Transplantable murine melanomas are well-established models for the study of experimental cancer therapies. Melanomas express different tumor-associated Ags, which are potential targets for novel designs of therapeutic cancer vaccines. Melanoma-associated Ags include tyrosinase and tyrosine-related protein (TRP)-1/2 or the MAGE and BAGE class of Ags. Many immunotherapeutic protocols have been tested using the murine B16 melanoma cell line (and its sublines) that originated in the syngeneic C57BL/6 (H-2b) mouse strain. B16 melanoma cells express some of these melanoma-associated Ags, and their growth can be controlled in vivo by different classes of specific or nonspecific immune effector cells. The B16 cell line contains a large amount of PDGF ß-receptor which makes it a suitable positive control for PDGF ß-receptor.

Storage:
Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Purity:
Whole Cell Lysate

Application:
Recommended dilutions
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.



1
 Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology.
2
 PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
3
 Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
4
 Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.