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K562

Cat. No.	Size	Price
M0060-100 ug	100 ug	$ 72.00
Full Name	K562

Species:

Human

Tissue:

mylogeneous leukemia

Description:

K562 whole cell lysate

Concentration:

Concentration: 100 μg/100 μl.
Storage buffer: whole cell lysate in 1 x SDS sample buffer containing 5% b-mercaptoethanol.

Volume:

20 ug per lane.

Storage:

Store at -20˚C for three months or at –70˚C for one year.

Background:

K562 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Application:

K562 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 ug per lane is recommended for mini gel.

EXAMPLE:

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