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Raji

Cat. No.	Size	Price
M0064-100 ug	100 ug	$ 71.50
Full Name	Raji

Species:

Human

Tissue:

B cell lymphoma

Description:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

Concentration:

Concentration: 100 μg/100 μl.
Storage buffer: Whole lysate in 1 X SDS 5% b-mercaptoethanol.

Note:

Cell line: Raji (Human Burkitt's lymphoma).
Growth media: RPMI & 10% NCS (Newborn calf serum).

Volume:

Raji cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane isrecommended for mini gel.

Storage:

Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Background:

Raji cell line is the first continuous human hematopoietic cell line. It has become an indispensable tool in haematological research and leukaemia and lymphoma studies. Raji cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

EXAMPLE:

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