You are here:  Products » Proteins, Expression, Isolation and Analysis » Protein Detection and Immunoassay  » Cell Lysates »  M0068

C2C12

Cat. No.	Size	Price
M0068-100 ug	100 ug	$ 72.00
Full Name	C2C12

Species:

Mouse

Tissue:

muscle

Description:

C2C12 (Mouse myoblast cell line) Whole cell lysate.

Concentration:

Concentration: 100 μg/100 μl.
Storage buffer: Whole cell lysate in 1 X SDS-PAGE sample loading buffer, with 5% b-mercaptoethanol.

Note:

Cell line: C2C12 (muscle; myoblast);
Growth media: DMEM & 10% fetal bovine serum.

Volume:

C2C12 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

Storage:

Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Background:

C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).Mouse C2C12 cell lysate was prepared by homogenization in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

EXAMPLE:

Zoom

1	Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology.
2	PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
3	Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
4	Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.