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A549

Cat. No.	Size	Price
M0076-100 ug	100 ug	$ 71.50
Full Name	A549

Species:

Human

Tissue:

lung

Description:

A549 whole cell lysate

Concentration:

Concentration: 100 μg/100 μl.
Storage buffer: Whole cell lysate in 1 x SDS sample buffer containing 5% b-mercaptoethanol.

Volume:

10 μg/lane.

Storage:

They are shipped on blue ice and can remain at –20oC for up to 3 months. For long term storage, keep lysates in dark at –70oC. Avoid repetitive freeze-thaw cycles, as this may result in degradatio

Background:

A549 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Application:

Imgenex lysates eliminate the time and hassle involved in preparing cell lysate. Each tissue or cell lysate is prepared in 1x sampling buffer at a concentration ideal for most SDS-PAGE applications. With Imgenex lysates, consistent quality protein separation is assured for several runs. One vial of Imgenex lysate is sufficient to run 10 lanes (10 μg/lane) of protein.

Related Products: Precast Gels Code Name Size Price   M0017 Lysates from Individual Cell Lines: H441 100 ug $71.50 M0018 Lysates from Individual Cell Lines: Calu-6 100 ug $71.50

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