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Mouse Anti-DYKDDDDK-tag Monoclonal Antibody, IgG1 Subclass

Note:
This product has been discontinued!
The improved replacement is  A01190

Catalog No. Size Price Figures
Full Name
AliasMouse Anti-DYKDDDDK-tag mAb
DescriptionMouse Anti-DYKDDDDK-tag Monoclonal Antibody is a purified IgG1/kappa monoclonal antibody isolated from murine ascites fluid that binds to proteins with a DYKDDDDK marker at a free N-terminus or C-terminus. It is useful for the identification of proteins with DYKDDDDK markers at free N-termini and C-termini by common immunological applications in yeast, animal, and E. coli cells. GenScript Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is also useful for affinity purification of proteins with a DYKDDDDK marker at free N- termini or C-termini when bound to a solid support.
Ig SubclassMouse IgG1/kappa
StorageStable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Stored at 4°C, the aliquot will remain stable for three months.
SpecificityRecognizes over-expressed proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-terminal of targeted proteins.
CloneA00013.01
ImmunogenA synthetic peptide (DYKDDDDK) coupled to KLH
ReconstitutionLyophilized from 1 mg/ml PBS solution. Reconstitute it with sterile water to 1 mg/ml.
ApplicationsWestern blot: 1:1,000
Immunoprecipitation: 1:200
Immunostaining: 1:200
ConjugationUnconjugated
Host SpeciesMouse
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical ManualTM0589, TM0590, TM0591, TM0592, TM0593
* For Non-Clinical Research Use Only *
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