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Mouse Anti-DYKDDDDK-tag Monoclonal Antibody, IgG1 Subclass

Note:
This product has been discontinued!
The improved replacement is  A01190

Catalog No. Size Price Figures
Full Name
AliasMouse Anti-DYKDDDDK-tag mAb
DescriptionMouse Anti-DYKDDDDK-tag Monoclonal Antibody is a purified IgG1/kappa monoclonal antibody isolated from murine ascites fluid that binds to proteins with a DYKDDDDK marker at a free N-terminus or C-terminus. It is useful for the identification of proteins with DYKDDDDK markers at free N-termini and C-termini by common immunological applications in yeast, animal, and E. coli cells. GenScript Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is also useful for affinity purification of proteins with a DYKDDDDK marker at free N- termini or C-termini when bound to a solid support.
Ig SubclassMouse IgG1/kappa
StorageStable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Stored at 4°C, the aliquot will remain stable for three months.
SpecificityRecognizes over-expressed proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-terminal of targeted proteins.
CloneA00013.01
ImmunogenA synthetic peptide (DYKDDDDK) coupled to KLH
ReconstitutionLyophilized from 1 mg/ml PBS solution. Reconstitute it with sterile water to 1 mg/ml.
ApplicationsWestern blot: 1:1,000
Immunoprecipitation: 1:200
Immunostaining: 1:200
ConjugationUnconjugated
Host SpeciesMouse
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
* For Non-Clinical Research Use Only *
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