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Mouse Anti-DYKDDDDK-tag Monoclonal Antibody

Catalog No. Size Price Figures
A00013-500 ug
500 ug
$ 220.00
MSDS:  20051003104711  (PDF)

EXAMPLE:
Mouse Anti-DYKDDDDK-tag (monoclonal) Western Blot
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References:
  • Schuster M, et al. Protein expression strategies for identification of novel target proteins. J Biomol Screen. Apr 2000; 5(2): 89-97.
  • Nielsen UB, et al. Targeting of bivalent anti-ErbB2 diabody antibody fragments to tumor cells is independent of the intrinsic antibody affinity. Cancer Res. Nov 2000; 60(22): 6434-6440.
  • Kimura M, et al. Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II. Mol Cell Biol. Mar 2002;22(5):1577-1588.
A00013-1 mg
1 mg
$ 410.00
Full Name
Mouse Anti-DYKDDDDK-tag Monoclonal Antibody
AliasDYKDDDDK-tag Monoclonal Antibody
DescriptionMouse Anti-DYKDDDDK-tag Monoclonal Antibody is a purified IgG1/kappa monoclonal antibody isolated from murine ascites fluid that binds to proteins with a DYKDDDDK marker at a free N-terminus or C-terminus. It is useful for the identification of proteins with DYKDDDDK markers at free N-termini and C-termini by common immunological applications in yeast, animal, and E. coli cells. GenScript Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is also useful for affinity purification of proteins with a DYKDDDDK markers at free N- termini or C-termini when bound to a solid support.
Ig SubclassMouse IgG1/kappa
StorageStable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Stored at 4°C, the aliquot will remain stable for three months.
SpecificityRecognises over-expressed proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-terminal of targeted proteins.
ConcentrationLyophilized powder. Reconstitute it with sterile water to 1 mg/ml.
CloneA00013.01
ImmunogenA synthetic peptide (DYKDDDDK) coupled to KLH
ApplicationsWestern blot: 1:1,000
Immunoprecipitation: 1:200
Immunostaining: 1:200
ConjugationUnconjugated
Host SpeciesMouse
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
* For Non-Clinical Research Use Only *
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A00160antibody : Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody1 mg
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