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The TM Anti-GST-tag Monoclonal Antibody (Mouse)-test  |
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Note: | This product has been discontinued! The improved replacement is A00865 |
| Catalog No. |
Size |
Price |
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| Full Name | | | Alias | Anti-GST-tag |
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| Abbreviated Name-2 | Anti-GST |
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| Description | Schistosoma Japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for the production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest. |
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| Ig Subclass | Mouse IgG2b |
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| Storage | Aliquots may be stored at -20°C or -80°C. Avoid repeated freeze/thaw cycles. After thawing, the product may be stored at 4°C for short periods. Aviod repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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| Specificity | TheTM Anti-GST-tag Monoclonal Antibody (Mouse) detects over expressed glutathione-transferase (GST) fusion proteins. |
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| Concentration | 1 mg/ml in PBS, pH 7.4 with 0.02% sodium azide. Frozen liquid. |
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| Clone | A00014.01 |
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| Immunogen | Recombinant GST protein |
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| Purification Method | Protein G affinity column |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.01-0.1 µg/ml
Western blot: 1.0 µg/ml |
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| Fusion Partner | Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells. |
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| Conjugation | Unconjugated |
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| Host Species | Mouse |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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| Technical Manual | TM0589, TM0590, TM0591, TM0592, TM0593 |
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| * For Non-Clinical Research Use Only *
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. | |
If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
