Immunoassay Products
You are here:  Products » Immunoassay Products » Catalog Antibody »  TheTM Anti-GST-tag Monoclonal Antibody (Mouse)-test   
 
  Search by Catalog Number:
   Search by Name:
   Search by Category:
 
TheTM Anti-GST-tag Monoclonal Antibody (Mouse)-test

Note:
This product has been discontinued!
The improved replacement is  A00865

Catalog No. Size Price Figures
Full Name
AliasAnti-GST-tag
Abbreviated Name-2Anti-GST
DescriptionSchistosoma Japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for the production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.
Ig SubclassMouse IgG2b
StorageAliquots may be stored at -20°C or -80°C. Avoid repeated freeze/thaw cycles. After thawing, the product may be stored at 4°C for short periods. Aviod repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
SpecificityTheTM Anti-GST-tag Monoclonal Antibody (Mouse) detects over expressed glutathione-transferase (GST) fusion proteins.
Concentration1 mg/ml in PBS, pH 7.4 with 0.02% sodium azide. Frozen liquid.
CloneA00014.01
ImmunogenRecombinant GST protein
Purification MethodProtein G affinity column
ApplicationsWorking concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.01-0.1 µg/ml
Western blot: 1.0 µg/ml
Fusion PartnerSpleen cells are fused with SP2/0-Ag14 mouse myeloma cells.
ConjugationUnconjugated
Host SpeciesMouse
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
* For Non-Clinical Research Use Only *
Related Products:
CodeNameSizePrice 
M0001Epitope Tag Positive Control Lysates: GST Lysates100 ug
$ 71.50
A00097 antibody : Rabbit Anti-GST-tag Polyclonal Antibody40 ug
$ 50.00
200 ug
$ 225.00
A00130antibody : Rabbit Anti-GST-tag [HRP] Polyclonal Antibody40 ug
$ 55.00
200 ug
$ 247.50
A00865 antibody : THETM Anti-GST Monoclonal Antibody (Mouse)40 ug
$ 69.00
200 ug
$ 311.00
A00202antibody : Rabbit Anti-GST-tag [Biotin] Polyclonal Antibody40 ug
$ 55.00
200 ug
$ 247.50
A00867 antibody : THETM Anti-GST-tag [Biotin] Monoclonal Antibody (Mouse)40 ug
$ 74.00
200 ug
$ 333.00
Z02039protein : Glutathione S-Transferase (GST)， Schistosoma japonicum1 mg
$ 70.00
L00208 Protein Expression and Purification Kit1 kit
$ 335.00



If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks.

Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance.