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Mouse Anti-Human Heparanase 1 (HPA1) Monoclonal Antibody  |
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Note: | This product has been discontinued!
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| Catalog No. |
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Price |
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| Full Name | | | Alias | Anti Human Heparanase 1 |
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| Abbreviated Name-2 | Anti Heparanase 1 |
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| Description | Heparanase is an endo-b-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1, 2, 3). Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4, 5). Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1, 6).
Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7, 8). |
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| Ig Subclass | Mouse IgG2Bk |
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| Storage | Store the product at 4°C. It will remain stable for six months after the date of shipment.
For extended storage, freeze in working aliquots at -20°C.
Avoid repeated freeze-thaw cycles. |
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| Specificity | HP3/17 reacts with the 50,000 Da subunit and with the 65,000 Da precursor of human or mouse heparanase by Western blotting and immunohistochemistry.
Recommended dilution range for Western blot analysis: 1:4,000
Recommended dilution range for immunohistochemistry: 1:40 |
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| Concentration | 100 ug/25 ul in 20 mM Sodium Phosphate buffer, 150 mM NaCl, pH 7.2, containing 0.001% Thimerosal.
Purity: >98% on SDS-PAGE when loaded 50 µg/lane. |
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| Clone | A00032.01 |
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| Applications | Western blot
Immunohistochemistry |
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| Conjugation | Unconjugated |
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| Host Species | Mouse |
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| Antigen Species | Human |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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| Technical Manual | TM0589, TM0590, TM0591, TM0592, TM0593 |
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| * For Non-Clinical Research Use Only *
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Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. | |
If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
