| Catalog No. |
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| Full Name | |
| Alias | Interleukin-10; Interleukin10; Mouse Anti-Human IL-10; Mouse Anti-Human IL10; |
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| Abbreviated Name-2 | mAHuIL-10; mAHuIL10 |
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| Description | IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. |
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| Ig Subclass | Mouse IgG1 |
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| Storage | The antibody may be stored in lyophilized form at 4°C in a dry environment for long periods. After reconstitution, aliquot and store any antibody not intended for use within one month at -20°C. |
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| Stability | The antibody will remain stable for two years if stored lyophilized and for one month if stored in solution at 4°C. |
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| Concentration | 1 mg/ml in PBS (after reconstitution) |
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| Clone | A00045.01 |
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| Immunogen | r.Human IL-10 |
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| Purification Method | Ion excahnge |
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| Reconstitution | Reconstitute with sterile H2O. Mix gently, wash the sides of the vial, and wait 30-60 seconds before use. |
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| Titer | By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
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| Applications | ELISAWestern blot
Immunoprecipitation
Immunohistochemistry
Intracellular staining |
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| Conjugation | Unconjugated |
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| Host Species | Mouse |
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| Antigen Species | Human |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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