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Rat Anti-Mouse B220 (B cell specific isoform of CD45) Monoclonal Antibody  |
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Note: | This product has been discontinued!
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| Full Name | | | Alias | Rat B220 |
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| Abbreviated Name-2 | rAmB220 |
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| Description | B220 (CD45R) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
B220 (CD45R) is required for t-cell activation through the antigen receptor. The first ptpase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. |
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| Ig Subclass | Rat IgM |
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| Storage | Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. |
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| Stability | Two years lyophilized, one month in solution at 4°C |
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| Concentration | 1 mg/ml in PBS (after reconstitution) |
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| Clone | A00060.01 |
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| Immunogen | Purified mouse LN B cells |
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| Purification Method | Ion exchange column |
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| Reconstitution | Reconstitute with H2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
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| Applications | Staining antibody. For staining, use 10 µl/106 cells. |
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| Conjugation | Unconjugated |
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| Host Species | Rat |
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| Antigen Species | Mouse |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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| * For Non-Clinical Research Use Only *
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