| Catalog No. |
Size |
Price |
Figures |
A00097-40 ug
| 40 ug | $ 50.00 | MSDS: 20080227223847 (PDF)
EXAMPLE:
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References:
- Kato T, et al. Frequency and significance of anti-glutathione S-transferase autoantibody (anti-GST A1-1) in autoimmune hepatitis. J. Autoimmun. May 2004; 22(3): 211-216.
- Morari EC, et al. Role of glutathione-S-transferase and codon 72 of P53 genotypes in epithelial ovarian cancer patients. J. Cancer Res. Clin. Oncol. Aug 2006; 132(8): 521-528.
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A00097-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | Rabbit Anti-GST-tag Polyclonal Antibody |
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| Alias | Rabbit Anti-GST-tag pAb |
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| Description | GenScript Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum. Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-GST-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
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| Specificity | This antibody is specific to the GST protein from Schistosoma japonicum and is useful for detecting GST and GST-fusion proteins. Cross-reactivity with cell lysate of E. coli bacteria, sf21 cell and yeast has not been observed. |
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| Concentration | 0.5 mg/ml in PBS, pH 7.4 with 0.02% sodium azide |
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| Immunogen | Recombinant Glutathione-S-Transferase from Schistosoma japonicum |
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| Purification Method | Immunoaffinity chromatography |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1 µg/ml
Western Blot Using One-Step WesternTM Kit: For quick results, GenScript One-Step WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Species Reactivity | Schistosoma japonicum |
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| Host Species | Rabbit |
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| Antigen Synonyms | Glutathione-S-Transferase Tag |
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| Antigen Species | Schistosoma japonicum |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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