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Rabbit Anti-GST-tag Polyclonal Antibody

Catalog No. Size Price Figures
A00097-40 ug
40 ug
$ 50.00
MSDS:  20080227223847  (PDF)

EXAMPLE:
Rabbit Anti-GST-tag Polyclonal Antibody One-Step Western bloting
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References:
  • Kato T, et al. Frequency and significance of anti-glutathione S-transferase autoantibody (anti-GST A1-1) in autoimmune hepatitis. J. Autoimmun. May 2004; 22(3): 211-216.
  • Morari EC, et al. Role of glutathione-S-transferase and codon 72 of P53 genotypes in epithelial ovarian cancer patients. J. Cancer Res. Clin. Oncol. Aug 2006; 132(8): 521-528.
A00097-200 ug
200 ug
$ 225.00
Full Name
Rabbit Anti-GST-tag Polyclonal Antibody
AliasRabbit Anti-GST-tag pAb
DescriptionGenScript Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum. Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted.
Ig SubclassRabbit IgG
StorageGenScript Rabbit Anti-GST-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
SpecificityThis antibody is specific to the GST protein from Schistosoma japonicum and is useful for detecting GST and GST-fusion proteins. Cross-reactivity with cell lysate of E. coli bacteria, sf21 cell and yeast has not been observed.
Concentration0.5 mg/ml in PBS, pH 7.4 with 0.02% sodium azide
ImmunogenRecombinant Glutathione-S-Transferase from Schistosoma japonicum
Purification MethodImmunoaffinity chromatography
ApplicationsWorking concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1 µg/ml
Western Blot Using One-Step WesternTM Kit: For quick results, GenScript One-Step WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized
ConjugationUnconjugated
Species ReactivitySchistosoma japonicum
Host SpeciesRabbit
Antigen SynonymsGlutathione-S-Transferase Tag
Antigen SpeciesSchistosoma japonicum
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
* For Non-Clinical Research Use Only *
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