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GST-tag Antibody, pAb, Rabbit  |
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| Catalog No. |
Size |
Price |
Figures |
A00097-40 ug
| 40 ug | $ 50.00 | MSDS: 20080227223847 (PDF)
COA: Rabbit Anti-GST-tag Polyclonal Antibody (PDF)
EXAMPLE:
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EXAMPLE:
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References:
- Kato T, et al. Frequency and significance of anti-glutathione S-transferase autoantibody (anti-GST A1-1) in autoimmune hepatitis. J. Autoimmun. May 2004; 22(3): 211-216.
- Morari EC, et al. Role of glutathione-S-transferase and codon 72 of P53 genotypes in epithelial ovarian cancer patients. J. Cancer Res. Clin. Oncol. Aug 2006; 132(8): 521-528.
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A00097-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | GST-tag Antibody, pAb, Rabbit |
| | Alias | Rabbit Anti-GST-tag pAb |
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| Description | GenScript Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum. Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-GST-tag Polyclonal Antibody is stable for two to three weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freeze and thaw cycles. |
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| Specificity | This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system
and other systems. |
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| Concentration | 0.5 mg/ml in PBS, pH 7.4 with 0.02% sodium azide |
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| Immunogen | Recombinant Glutathione-S-Transferase from Schistosoma japonicum |
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| Purification Method | Immunoaffinity chromatography |
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| Applications | Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Species Reactivity | Schistosoma japonicum |
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| Host Species | Rabbit |
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| Antigen Synonyms | Glutathione-S-Transferase Tag |
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| Antigen Species | Schistosoma japonicum |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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| * For Non-Clinical Research Use Only *
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. | |
If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
