| Catalog No. |
Size |
Price |
Figures |
A00100-100 ug
| 100 ug | $ 203.50 | MSDS: 20081019230027 (PDF)
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References:
- Featherstone C, et al. A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo. J. Cell Biol. Oct 1988; 107(4): 1289-1297.
- Aris JP, Blobel G. Yeast nuclear envelope proteins cross react with an antibody against mammalian pore complex proteins. J. Cell Biol. Jun 1989; 108(6): 2059-2067.
- Snow CM, et al. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J. Cell Biol. May 1987; 104(5): 1143-1156.
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| Full Name | | Mouse Anti-Yeast Nuclear Pore Complex Monoclonal Antibody |
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| Alias | Mouse Anti-Yeast Nuclear Pore Complex mAb |
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| Abbreviated Name-2 | Anti-Yeast Nuclear Pore Complex (NPC) |
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| Description | The nuclear pore complex (NPC) has a molecular mass of ≈125,000 Da in vertebrates and contains about 50 or more different proteins. The NPC spans the dual membrane of the nuclear envelope (NE) and acts as a gateway for macromolecular traffic between the cytoplasm and the nucleus. The basic framework of the NPC consists of a central core with a ring-spoke structure exhibiting eightfold radial symmetry. From this central ring 50 to 100 nm fibrils extend into the nucleoplasm and the cytoplasm. The NPC is in turn anchored in the NE by the nuclear lamina, a meshwork of lamins and lamin-associated proteins that forms a 15 nm thick fibrous structure between the inner nuclear membrane and peripheral chromatin. A number of proteins called nucleoporins (Nups) have been localized to discrete regions of the NPC and are often used as markers for this compartment, e.g. Nup153. Approximately half of the nucleoporins contain a phenyalanine-glycine repeat motif (FG repeat), which may be diagnostic for proteins playing a role in nuclear transport. This is a reliable general purpose monoclonal antibody that recognizes a related family of NPC proteins. Mouse Anti-Yeast Nuclear Pore Complex is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis. |
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| Ig Subclass | Mouse monoclonal (IgG1) |
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| Storage | Mouse Anti-Yeast Nuclear Pore Complex mAb will remain stable for one year from the date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquots will remain stable for three months if stored at 4°C. |
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| Specificity | Panspecific nuclear pore complex protein, useful nuclear pore complex and nuclear envelope marker in all eukaryotes tested to date |
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| Concentration | 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid |
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| Clone | A00100.01 |
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| Immunogen | Mouse Anti-Yeast Nuclear Pore Complex mAb is raised by injecting mice with yeast nuclear preparations and screening the resulting hydridomas by immunfluoscence on yeast cells. |
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| Purification Method | Immunoaffinity purification |
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| Applications | Immunofluorescence on yeast cells: 1:50-1:250
Immunofluorescence on mammalian cells: 1:25-1:50 |
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| Conjugation | Unconjugated |
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| Host Species | Mouse |
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| Antigen Species | Yeast |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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