| Catalog No. |
Size |
Price |
Figures |
A00105-100 ug
| 100 ug | $ 203.50 | MSDS: 20081019231912 (PDF)
EXAMPLE:
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References:
- Pintard L, et al. Spb1p is a yeast nucleolar protein associated with Nop1p and Nop58p that is able to bind S-adenosyl-L-methionine in vitro. Feb 2000; 20(4): 1370-1381.
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| Full Name | | Mouse Anti-Nop1p Monoclonal Antibody |
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| Alias | Mouse Anti-Nop1p Monoclonal Antibody |
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| Abbreviated Name-2 | Anti Nop1p |
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| Description | Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal -80 amino acids contain multiple copies based on the peptide RGG and the remaining ~240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 alpha helixes. Patients with the autoimmune disease scleroderma often have circulating auto-antibodies to a 34,000 Da protein which was subsequently found to be fibrillarin. |
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| Storage | Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months. |
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| Specificity | Recognises Yeast (S. cerevisiae) Nop1p. This monoclonal antibody does not cross-react with fibrillarin in other species, but is an excellent marker of the nucleous or yeast cells. |
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| Concentration | 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid |
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| Clone | A00105.01 |
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| Immunogen | Yesat (S. cerevisiae) fibrillarin |
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| Purification Method | Immunoaffinity purification |
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| Applications | Western blots of yeast protein samples: 1:5,000 (cell lysates) to 1:12,500 (nuclear fractions), followed by chemiluminescent detection (ECL)
Other (non-ECL) Western detection methods: 1:500 to 1:2,500
Immunofluoscence on yeast cells: 1:1,000 to 1:10,000 |
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| Conjugation | Unconjugated |
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| Species Reactivity | Yeast |
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| Host Species | Mouse |
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| Antigen Species | Yeast |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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