| Catalog No. |
Size |
Price |
Figures |
A00133-100 ug
| 100 ug | $ 203.50 | MSDS: 20081020045654 (PDF)
References:
- von Mikecz A. The nuclear ubiquitin-proteasome system.J. Cell. Sci. May 2006; 119(Pt 10): 1977-1984.
- Xu G, et al. Post-translational regulation of adipose differentiation-related protein by the ubiquitin/proteasome pathway. J. Biol. Chem. Dec 2005; 280(52): 42841-42847.
- Jain M, Das RH. Identification and expression of a conserved ubiquitin gene homologue of Spodoptera litura nucleopolyhedrovirus (spltNPV-I). Mol. Biol. Rep. Dec 2004; 31(4): 261-266.
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| Full Name | | Rabbit Anti-Ubiquitin Polyclonal Antibody |
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| Alias | Rabbit Anti-Ubiquitin pAb |
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| Abbreviated Name-2 | Anti Ubiquitin |
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| Description | Ubiquitin is a highly conserved protein of about 8,500 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies. |
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| Ig Subclass | Rabbit IgG |
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| Storage | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months. |
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| Specificity | Detects ubiquitinated inclusion bodies such as neurofibrillary tangles of Alzheimer's disease, Lewy bodies of Parkinson's disease, Pick bodies of Pick's disease. Works well on formalin fixed material. |
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| Concentration | 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid |
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| Immunogen | Glutaraldehyde cross linked ubiquitin in rabbit |
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| Purification Method | Immunoaffinity purification |
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| Applications | Immunofluorescence: 1:25 to 1:500
Western blot: 1:1,000 |
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| Conjugation | Unconjugated |
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| Host Species | Rabbit |
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| Antigen Species | Rabbit |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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