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His-tag Antibody, pAb, Rabbit  |
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| Catalog No. |
Size |
Price |
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A00174-40 ug
| 40 ug | $ 50.00 | MSDS: 20080228011555 (PDF)
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A00174-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | His-tag Antibody, pAb, Rabbit |
| | Alias | Rabbit Anti-His-Tag |
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| Abbreviated Name-2 | Anti-His-Tag |
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| Description | Rabbit Anti-His-tag Polyclonal Antibody recognizes the His tag placed at N-terminal, C-terminal, and internal regions of targeted proteins in transfected or transformed cells. Short-sequence epitope tags are common in the study of protein expression in several different expression systems. In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-His-tag Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles. |
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| Specificity | GenScript Rabbit Anti-His-tag Polyclonal Antibody specifically reacts with fusion proteins containing either amino- or carboxy-terminal His epitope tags. |
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| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
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| Immunogen | His epitope tag peptide HHHHHH conjugated to KLH |
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| Purification Method | Immunoaffinity chromatography |
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| Reconstitution | Reconstitute the lyophilized powder with 80 µl of deionized water (or equivalent) to antibody concentration of 0.5 mg/ml. |
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| Applications | Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Host Species | Rabbit |
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| Note | Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
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| * For Non-Clinical Research Use Only *
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. | |
If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
