| Catalog No. |
Size |
Price |
Figures |
A00174-40 ug
| 40 ug | $ 50.00 | MSDS: 20080228011555 (PDF)
EXAMPLE:
Zoom
EXAMPLE:
Zoom
EXAMPLE:
Zoom
EXAMPLE:
Zoom
|
|---|
A00174-200 ug
| 200 ug | $ 225.00 |
|---|
| Full Name | | Rabbit Anti-His-tag Polyclonal Antibody |
|
| Alias | Rabbit Anti-His-Tag |
|---|
| Abbreviated Name-2 | Anti-His-Tag |
|---|
| Description | Rabbit Anti-His-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. |
|---|
| Ig Subclass | Rabbit IgG |
|---|
| Storage | GenScript Rabbit Anti-His-tag Polyclonal Antibody remains stable for 2-3 weeks if stored at 2-8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. |
|---|
| Specificity | GenScript Rabbit Anti-His-tag Polyclonal Antibody specifically reacts with over-expressed fusion proteins containing either amino- or carboxy-terminal His epitope tags. |
|---|
| Concentration | 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide |
|---|
| Immunogen | His epitope tag peptide HHHHHH conjugated to KLH |
|---|
| Purification Method | Immunoaffinity purified |
|---|
| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using One-Step WesternTM Kit: For quick results, GenScript One-Step WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized |
|---|
| Conjugation | Unconjugated |
|---|
| Host Species | Rabbit |
|---|
| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
|---|
