| Catalog No. |
Size |
Price |
Figures |
A00174-40 ug
| 40 ug | $ 50.00 | MSDS: 20080228011555 (PDF)
MSDS: 20051008215330 (PDF)
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A00174-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | Rabbit Anti-His-tag Polyclonal Antibody |
|
| Alias | Rabbit Anti-His-Tag |
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| Abbreviated Name-2 | Anti-His-Tag |
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| Description | Rabbit Anti-His-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-His-tag Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles. |
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| Specificity | GenScript Rabbit Anti-His-tag Polyclonal Antibody specifically reacts with fusion proteins containing either amino- or carboxy-terminal His epitope tags. |
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| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
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| Immunogen | His epitope tag peptide HHHHHH conjugated to KLH |
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| Purification Method | Immunoaffinity chromatography |
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| Reconstitution | Reconstitute the lyophilized powder with 80 µl of deionized water (or equivalent) to antibody concentration of 0.5 mg/ml. |
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| Applications | Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Host Species | Rabbit |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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