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Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb

Catalog No. Size Price Figures
A00178-1 mg
1 mg
$ 63.00
MSDS:  20080228012329  (PDF)
MSDS:  20090114012119  (PDF)

Full Name
Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb
AliasDonkey Anti-Goat IgG (H&L) [HRP]
Abbreviated Name-2Anti-Goat IgG (H&L) [HRP]
DescriptionDonkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody is highly purified from donkey antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with goat IgG heavy and light chains.
Ig SubclassDonkey IgG
StorageGenScript Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.
Concentration1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
ImmunogenFull-length goat IgG (purified)
Purification MethodImmunoaffinity chromatography
ReconstitutionReconstitute the lyophilized powder with 1 ml of deionized water (or equivalent) to antibody concentration of 1 mg/ml.
ApplicationsDonkey Anti-Goat IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:100,000
Western blot: 1:1,000-1:20,000
Other applications: user-optimized
ConjugationHorseradish peroxidase (HRP)
Species ReactivityGoat
Host SpeciesDonkey
Antigen SpeciesGoat
NoteSmall volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.
* For Non-Clinical Research Use Only *


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