| Catalog No. |
Size |
Price |
Figures |
A00178-1 mg
| 1 mg | $ 63.00 | MSDS: 20080228012329 (PDF)
MSDS: 20090114012119 (PDF)
|
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| Full Name | | Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody |
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| Alias | Donkey Anti-Goat IgG (H&L) [HRP] |
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| Abbreviated Name-2 | Anti-Goat IgG (H&L) [HRP] |
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| Description | Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody is highly purified from donkey antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with goat IgG heavy and light chains. |
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| Ig Subclass | Donkey IgG |
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| Storage | Donkey Anti-Goat IgG (H&L) [HRP] should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for 2-3 weeks 2-8°C or for up to two years at -20°C or below. Avoid repeated freezing and thawing cycles. |
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| Concentration | 1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
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| Immunogen | Full-length goat IgG (purified) |
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| Purification Method | Immunoaffinity chromatography |
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| Reconstitution | Reconstitute the lyophilized powder with 1 ml of deionized water (or equivalent) |
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| Applications | Donkey Anti-Goat IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:100,000
Western blot: 1:1,000-1:20,000
Other applications: user-optimized |
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| Conjugation | Horseradish peroxidase (HRP) |
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| Species Reactivity | Goat |
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| Host Species | Donkey |
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| Antigen Species | Goat |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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