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Trx-tag Antibody, mAb, Mouse

Catalog No. Size Price Figures
A00180-40 ug
 		40 ug
$ 50.00
MSDS:  20080228012615  (PDF)

EXAMPLE:
Mouse Anti Trx-tag (monoclonal) Western blot
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EXAMPLE:
Mouse Anti-Trx-tag Monoclonal Antibody one-step WB
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A00180-200 ug
 		200 ug
$ 225.00
Full Name
Trx-tag Antibody, mAb, Mouse
AliasMouse Anti-Trx-tag
Abbreviated Name-2Anti-Trx-tag
DescriptionMouse Anti-Trx-tag Monoclonal Antibody reacts with the 109-aa Trx-tag sequence encoded by the PET-32a expression vectors from Novagen. Mouse Anti-Trx-tag Monoclonal Antibody is suitable for detecting fusion proteins that contain a Trx-tag.
Key Features
  • GenScript Trx-tag antibody has high sensitivity.
  • GenScript Trx-tag antibody can recognize N-terminal, C-terminal and internal Trx-tag in fusion proteins.
    • Ig SubclassMouse IgG2a
    StorageStore at 4°C short term. Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
    SpecificityThis antibody reacts with the Thioredoxin fusion protein from E. coli and human. Not yet tested in other species.
    Concentration1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
    Clone4C12H10
    ImmunogenPurified recombinant Trx-tag fusion protein
    Purification MethodProtein G affinity column
    ApplicationsWorking concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.1-1.0 µg/ml
    Western blot: 1.0 µg/ml
    Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2ml of PBST).
    Other applications: user-optimized
    Fusion PartnerSpleen cells were fused with SP2/0-Ag14 mouse myeloma cells
    ConjugationUnconjugated
    Species ReactivityE. coli and human
    Host SpeciesMouse
    NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
    * For Non-Clinical Research Use Only *
    Related Products:
    CodeNameSizePrice 
    A00129antibody : Trx-tag Antibody, pAb, Goat40 ug
    $ 50.00
    200 ug
    $ 225.00
    A00160 antibody : Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb 1 mg
    $ 60.00




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