| Catalog No. |
Size |
Price |
Figures |
A00185-40 ug
| 40 ug | $ 50.00 | MSDS: 20080228013416 (PDF)
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EXAMPLE:
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EXAMPLE:
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A00185-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | Mouse Anti-cGFP-tag Monoclonal Antibody |
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| Alias | Anti-cGFP-tag |
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| Description | The coral green fluorescent protein (cGFP) from vector pRNATin-H1.2/Neo (GenScript, SD1223) is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. Mouse Anti-cGFP-tag Monoclonal Antibody can be used to detect cGFP fusion protein in Western blot analyses. |
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| Key Features | GenScript cGFP-tag antibody has high sensitivity.
GenScript cGFP-tag antibody can recognize various forms of GFP proteins including eGFP, OFP and GFPuv. |
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| Ig Subclass | Mouse IgG2b |
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| Storage | The antibody may be store at 4°C for short term periods. Store for long periods at -20°C or -80°C for longer periods. Avoid repeated freeze/thaw cycles. |
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| Specificity | The antibody reacts with various forms of GFP including eGFP, OFP, and GFPuv. |
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| Concentration | 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide |
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| Clone | A00185.01 |
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| Immunogen | Purified recombinant cGFP fusion protein |
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| Purification Method | Protein G affinity column |
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| Applications | The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and length of incubations
ELISA: 0.1-1.0 µg/ml
Western blot: 1.0-2.0 µg/ml
Western blot using One-Step WesternTM Kit: For quick results, GenScript One-Step WesternTM Kit L00205C is recommended. 4 µg of this antibody is mixed with 4 ml of WB solution.
Other applications: user-optimized |
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| Fusion Partner | Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells. |
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| Conjugation | Unconjugated |
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| Host Species | Mouse |
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| Antigen Synonyms | Coral green fluorescent protein |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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