| Catalog No. |
Size |
Price |
Figures |
A00186-100 ug
| 100 ug | List Price: $ 535.00
Promotion Price: $ 374.50 | PROTOCOL: 20070630080710 (PDF)
MSDS: 20080418041037 (PDF)
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EXAMPLE:
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EXAMPLE:
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| Full Name | | THETM Anti-His Monoclonal Antibody |  |
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| Alias | Anti-His mAb |
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| Description | Monoclonal antibodies specific to polyhistidine can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag. THETM Anti-His mAb (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4XHis- and 5XHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody. THETM Anti-His mAb is produced from mice ascites and purified by protein G affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM Anti-His mAb recognizes polyhistidine tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. THETM Anti-His mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells. |
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| Ig Subclass | Mouse IgG1 |
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| Storage | THETM Anti-His mAb should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored 2-8°C. The reconstituted antibody can be stored for up to three months 2-8°C or for up to two years at or below -20°C. Avoid repeated freezing and thawing cycles. |
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| Specificity | The antibody recognizes C-terminal, N-terminal, and internal tagged
fusion proteins. |
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| Concentration | 100 μg, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
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| Clone | A00186.01 |
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| Immunogen | Synthetic peptide containing six histidine residues (6xHis) coupled to KLH |
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| Purification Method | Protein G affinity column |
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| Reconstitution | Reconstitute lyophilized THETM His mAb (100 μg) with 100 μl of deionized water to a final antibody concentration of 1 mg/ml. |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-0.2 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Immunofluorescent staining: 1 µg/ml
FAC assay/Flow cytometry: 1 µg/ml
Other applications: user-optimized |
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| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells. |
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| Conjugation | Unconjugated |
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| Host Species | Mouse |
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| Antigen Synonyms | Histidine |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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