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| Goat Anti-GAPDH [HRP] Polyclonal Antibody |
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| Catalog No. |
Size |
Price |
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A00192-40 ug
| 40 ug | $ 60.00 | EXAMPLE:
![Goat Anti-GAPDH [HRP] Polyclonal Antibody Western blot](http://www.genscript.com/site2/document/3741_20080122030516.JPG "Goat Anti-GAPDH [HRP] Polyclonal Antibody Western blot")
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EXAMPLE:
![Goat Anti-GAPDH [HRP] Polyclonal Antibody](http://www.genscript.com/site2/document/3849_20080131035852.JPG "Goat Anti-GAPDH [HRP] Polyclonal Antibody")
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A00192-200 ug
| 200 ug | $ 270.00 |
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| Full Name | | Goat Anti-GAPDH [HRP] Polyclonal Antibody |
| | Alias | Anti-GAPDH [HRP] |
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| Description | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. Though expressed differently in different tissues, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often measured as a control in experiments quantifying specific changes in expression of other targets. Recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntington's and Alzheimer's disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
GenScript Goat Anti-GAPDH [HRP] Polyclonal Antibody is developed in goat using GAPDH protein from rabbit muscle. This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml. |
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| Ig Subclass | Goat IgG |
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| Storage | GenScript Goat Anti-GAPDH [HRP] Polyclonal Antibody remains stable for 2-3 weeks if stored at 2-8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript Goat Anti-GAPDH [HRP] Polyclonal Antibody specifically reacts with GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell and tissue lysates. It has not yet been tested with other species. |
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| Concentration | 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol,and 0.01% thimerosal |
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| Immunogen | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle |
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| Purification Method | Immunoaffinity chromatography |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized |
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| Conjugation | Horseradish peroxidase (HRP) |
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| Species Reactivity | Human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli |
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| Antigen Synonyms | Glyceraldehyde-3-phosphate dehydrogenase |
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| Note | Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
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| * For Non-Clinical Research Use Only * If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
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