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GAPDH Antibody [HRP], pAb, Goat

Catalog No. Size Price Figures
A00192-40 ug
40 ug
$ 60.00
MSDS:  20081021024451  (PDF)
MSDS:  20090114012119  (PDF)

EXAMPLE:
Goat Anti-GAPDH [HRP] Polyclonal Antibody Western blot
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EXAMPLE:
Goat Anti-GAPDH [HRP] Polyclonal Antibody
Zoom
EXAMPLE:
Goat Anti-GAPDH [HRP] Polyclonal Antibody WB
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A00192-200 ug
200 ug
$ 270.00
Full Name
GAPDH Antibody [HRP], pAb, Goat
AliasAnti-GAPDH [HRP]
DescriptionGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. Though expressed differently in different tissues, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often measured as a control in experiments quantifying specific changes in expression of other targets. Recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntingtons and Alzheimers disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Ig SubclassGoat IgG
StorageThe antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
SpecificityGenScript Goat Anti-GAPDH [HRP] Polyclonal Antibody specifically reacts with GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell and tissue lysates. It has not yet been tested with other species.
Concentration0.5 mg/ml, lyophilized with PBS, pH7.4, containing 1%BSA, 0.01% thimerosal
ImmunogenGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle
Purification MethodImmunoaffinity chromatography
ReconstitutionReconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml.
ApplicationsWorking concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
ConjugationHorseradish peroxidase (HRP)
Species ReactivityHuman, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli
Antigen SynonymsGlyceraldehyde-3-phosphate dehydrogenase
NoteSmall volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.
Technical ManualTM0589TM0590TM0591TM0592TM0593
* For Non-Clinical Research Use Only *
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A00191antibody : GAPDH Antibody, pAb, Goat40 ug
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