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NF-κB p65 Antibody (Phospho-Ser529), pAb, Rabbit

Catalog No. Size Price Figures
A00330-40 ug
 		40 ug
$ 69.00
MSDS:  20081029025224  (PDF)

EXAMPLE:
Rabbit Anti NF-κB p65 (Phospho-Ser529) (polyclonal) Western Blot analysis
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EXAMPLE:
Rabbit Anti-NF-κB p65 (Phospho-Ser529) Polyclonal Antibody IHC
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EXAMPLE:
Rabbit Anti-NF-κB p65 (Phospho-Ser529) Polyclonal Antibody Immunofluorescence Analysis
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A00330-200 ug
 		200 ug
$ 311.00
Full Name
NF-κB p65 Antibody (Phospho-Ser529), pAb, Rabbit
AliasAnti-NF-κB p65 (Phospho-Ser529)
Ig SubclassRabbit IgG
StorageStore the antibody at -20°C. It will remain stable for one year.
SpecificityGenScript detects endogenous levels of NF-κB p65 protein only when phosphorylated at serine 529.
Concentration1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
ImmunogenSynthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 529 (L-L-SP-G-D)
Purification MethodThe antibody is affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
ApplicationsWestern blot: 1:500-1:1,000
IHC: 1:50-1:100
IF: 1:100-1:200
ConjugationUnconjugated
Species ReactivityHuman, mouse, rat
Host SpeciesRabbit
Antigen SpeciesHuman
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical ManualTM0252, TM0253, TM0254
* For Non-Clinical Research Use Only *
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