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HSP27 Antibody (Phospho-Ser15), pAb, Rabbit

Catalog No. Size Price Figures
A00343-40 ug
40 ug
$ 69.00
MSDS:  20081029034858  (PDF)

EXAMPLE:
Rabbit Anti HSP27 (Phospho-Ser15) (polyclonal) Immunohistochemical analysis
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EXAMPLE:
Rabbit Anti HSP27 (Phospho-Ser15) (polyclonal) Western blot analysis
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EXAMPLE:
Rabbit Anti-HSP27 (Phospho-Ser15) Polyclonal Antibody IF
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A00343-200 ug
200 ug
$ 311.00
Full Name
HSP27 Antibody (Phospho-Ser15), pAb, Rabbit
AliasAnti-HSP27 (Phospho-Ser15)
Ig SubclassRabbit IgG
StorageStore the antibody at -20°C. It will remain stable for one year if stored properly.
SpecificityGenScript Rabbit Anti-HSP27 (Phospho-Ser15) Polyclonal Antibody detects endogenous levels of HSP27 only when phosphorylated at serine 15.
Concentration1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
ImmunogenSynthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 15 (G-P-SP-W-D)
Purification MethodThe antibody is affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
ApplicationsWestern blot: 1:500-1:1,000
IHC: 1:50-1:100
IF: 1:100-1:200
ConjugationUnconjugated
Species ReactivityHuman
Host SpeciesRabbit
Antigen SpeciesHuman
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical ManualTM0252TM0253TM0254
* For Non-Clinical Research Use Only *
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