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Rabbit Anti-HAT-tag Polyclonal Antibody

Catalog No. Size Price Figures
A00636-40 ug
40 ug
$ 50.00
MSDS:  20080228011150  (PDF)

EXAMPLE:
Rabbit Anti-HAT-tag Polyclonal Antibody, western blot analysis
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A00636-200 ug
200 ug
$ 225.00
Full Name
Rabbit Anti-HAT-tag Polyclonal Antibody
AliasRabbit Anti-HAT-tag pAb
DescriptionGenScript Rabbit Anti-HAT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.
Ig SubclassRabbit IgG
StorageGenScript Rabbit Anti-HAT-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
SpecificityGenScript Rabbit Anti-HAT-tag Polyclonal Antibody specifically reacts with over-expressed fusion proteins containing HAT epitope tag.
Concentration1 mg/ml in PBS, pH7.4, containing 0.02% sodium azid
ImmunogenHAT epitope tag peptide KDHLIHNVHKEFHAHAHNK conjugated to KLH
Purification MethodImmunoaffinity-purified
ApplicationsWorking concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using One-Step WesternTM Kit: For quick results, GenScript One-Step WesternTM Kit L00204C is recommended. 5 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
ConjugationUnconjugated
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
* For Non-Clinical Research Use Only *

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