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| Rabbit Anti-B-tag Polyclonal Antibody |
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| Catalog No. |
Size |
Price |
Figures |
A00638-40 ug
| 40 ug | $ 50.00 | EXAMPLE:
Zoom
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A00638-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | Rabbit Anti-B-tag Polyclonal Antibody |
| | Alias | Rabbit Anti-B-tag |
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| Abbreviated Name-2 | Anti-B-tag |
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| Description | Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The B-tag is an epitope tag composed of a six-residue peptide, QYPALT, corresponding to a highly conserved region of VP7 core protein of the blue tongue virus. The GenScript Rabbit Anti-B-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic B-tag peptide conjugated to KLH.
GenScript Rabbit Anti-B-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-B-tag Polyclonal Antibody remains stable for 2-3 weeks if stored at 2-8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript Rabbit Anti-B-tag Polyclonal Antibody specifically binds to fusion proteins containing B epitope tag. |
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| Concentration | 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide |
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| Immunogen | B epitope tag peptide QYPALT conjugated to KLH |
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| Purification Method | Immunoaffinity |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using the One-Step WesternTM Kit: For quick results, the GenScript One-Step WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for an 8 cm X 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Host Species | Rabbit |
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| Note | Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap. |
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| * For Non-Clinical Research Use Only * If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
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