| |
| Rabbit Anti-SUMO-tag Polyclonal Antibody |
|
|
| Catalog No. |
Size |
Price |
Figures |
A00640-500 ug
| 500 ug | $ 145.00 | MSDS: 20080228013647 (PDF)
EXAMPLE:
Zoom
EXAMPLE:
Zoom
EXAMPLE:
Zoom
|
|---|
| Full Name | | Rabbit Anti-SUMO-tag Polyclonal Antibody |
| | Alias | Rabbit Anti-SUMO-tag pAb |
|---|
| Description | Small ubiquitin-related modifier (SUMO) proteins are conjugated to numerous intracellular targets and serve to modulate protein interaction, localization, activity, and stability. SUMO (also known as 'Smt3' and 'sentrin' in other organisms) is linked to several different pathways, including nucleocytoplasmic transport. The attachment of SUMO to targets proteins is stimulated by (protein inhibitor of activated STATs PIAS) proteins that serve as E3-like ligases.
GenScript Rabbit Anti-SUMO-tag Polyclonal Antibody is developed in rabbit s using purified recombinant yeast SUMO protein. This polyclonal antibody is highly purified from rabbit antiserum by protein G chromatography and is supplied as a 200 μg aliquot at a concentration of 5 mg/ml. |
|---|
| Ig Subclass | Rabbit IgG |
|---|
| Storage | GenScript Rabbit Anti-SUMO-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
|---|
| Specificity | Rabbit Anti-SUMO-tag Polyclonal Antibody is specific to fusion proteins containing SUMO protein. |
|---|
| Concentration | 5 mg/ml in PBS, pH7.4, containing 0.02% sodium azide |
|---|
| Immunogen | Purified recombinant yeast SUMO |
|---|
| Purification Method | Protein G chromatography |
|---|
| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:5,000-1:20,000
Western blot: 1:1,000-1:10,000
Western blot using One-Step WesternTM Kit: For quick results, GenScript One-Step WesternTM Kit L00204C is recommended. 10 µl of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized |
|---|
| Conjugation | Unconjugated |
|---|
| Host Species | Rabbit |
|---|
| Antigen Species | Yeast |
|---|
| Note | Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
|---|
| * For Non-Clinical Research Use Only * If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
|
