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AMPKα1/AMPKα2 Antibody (Ab-174/172), pAb, Rabbit

Catalog No. Size Price Figures
A00664-40 ug
40 ug
$ 50.00
MSDS:  20080118034804  (PDF)
MSDS:  20081106025716  (PDF)

EXAMPLE:
Rabbit Anti-AMPK1 (Ab-174) Polyclonal Antibody IHC
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EXAMPLE:

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A00664-200 ug
200 ug
$ 225.00
Full Name
AMPKα1/AMPKα2 Antibody (Ab-174/172), pAb, Rabbit
AliasAMPKα1/AMPKα2 Antibody (Ab-174/172), pAb, Rabbit
DescriptionResponsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-COA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-COA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Ig SubclassRabbit IgG
StorageStore at -20°C/1 year.
SpecificityGenScript AMPKα1/AMPKα2 Antibody (Ab-174/172) detects endogenous levels of total AMPKα1/AMPKα2 protein.
Concentration1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
ImmunogenSynthesized non-phosphopeptide derived from human AMPKα1/AMPKα2 around the phosphorylation site of threonine 174/172 (L-R-TP-S-C).
Purification MethodGenScript Rabbit Anti-AMPKα1/AMPKα2 Antibody (Ab-174/172) Polyclonal Antibody was purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsIHC: 1:50-1:100
WB: 1:500-1:1,000
IF: 1:100-1:200
ConjugationUnconjugated
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Antigen SpeciesHuman
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical ManualTM0589TM0590TM0591TM0592TM0593
* For Non-Clinical Research Use Only *
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