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| Rabbit Anti-Avi-tag Polyclonal Antibody |
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| Catalog No. |
Size |
Price |
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A00674-40 ug
| 40 ug | $ 50.00 | MSDS: 20080228024932 (PDF)
EXAMPLE:
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A00674-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | Rabbit Anti-Avi-tag Polyclonal Antibody |
| | Alias | Rabbit Anti-Avi-tag pAb |
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| Abbreviated Name-2 | Rabbit Anti-Avi-tag |
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| Description | Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.
The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. GenScript Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.
This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 100 μl aliquot at a concentration of 1 mg/ml in PBS, pH7.4, containing 0.02% sodium azide. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-Avi-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript Rabbit Anti-Avi-tag Polyclonal Antibody is specific to fusion proteins containing Avi epitope tag. |
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| Purification Method | Immunoaffinity chromatography |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and time of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using One-Step WesternTM Kit: For quick results, GenScript One-Step WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Host Species | Rabbit |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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| * For Non-Clinical Research Use Only * If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
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