| Catalog No. |
Size |
Price |
Figures |
A00676-40 ug
| 40 ug | $ 50.00 | EXAMPLE:
Zoom
EXAMPLE:
Zoom
|
|---|
A00676-200 ug
| 200 ug | $ 225.00 |
|---|
| Full Name | | Rabbit Anti-DDDDK-tag Polyclonal Antibody |
|
| Alias | Rabbit Anti-DDDDK-tag pAb |
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| Abbreviated Name-2 | Anti-DDDDK-tag pAb |
|---|
| Description | Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The xxxDDDDK-tag is a small size and high hydrophilicity epitope tag containing an enterokinase cleavage site that allows it to be completely removed from the fusion proteins.
The GenScript Rabbit Anti-DDDDK-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide containing xxxDDDDK epitope conjugated to KLH.
This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-DDDDK-tag remains stable for 2-3 weeks if stored at 2-8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript Rabbit Anti-DDDDK-tag recognizes the enterokinase cleavage site and specifically binds to over-expressed fusion proteins containing xxxDDDDK epitope tags, such as Xpress tag. |
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| Concentration | 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide |
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| Immunogen | A synthetic epitope peptide xxxDDDDK conjugated to KLH. This sequence represents the enterokinase cleavage site (ECS). |
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| Purification Method | Immunoaffinity |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using the One-Step WesternTM Kit: For quick results, One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C) is recommended. 5 µg of this antibody is mixed with 10 ml of WB solution for any 8 cm X 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Host Species | Rabbit
|
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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