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DDDDK-tag Antibody, pAb, Rabbit

Catalog No. Size Price Figures
A00676-40 ug
40 ug
$ 50.00
MSDS:  20081107004754  (PDF)

EXAMPLE:
Rabbit Anti-Xpress-tag Polyclonal Antibody western blot analysis
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EXAMPLE:
Rabbit Anti-Xpress-tag Polyclonal Antibody western blot analysis
Zoom
A00676-200 ug
200 ug
$ 225.00
Full Name
DDDDK-tag Antibody, pAb, Rabbit
AliasRabbit Anti-DDDDK-tag pAb
Abbreviated Name-2Anti-DDDDK-tag pAb
DescriptionWell-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The xxxDDDDK-tag is a small size and high hydrophilicity epitope tag containing an enterokinase cleavage site that allows it to be completely removed from the fusion proteins.
The GenScript Rabbit Anti-DDDDK-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide containing xxxDDDDK epitope conjugated to KLH.
This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml.
Ig SubclassRabbit IgG
StorageGenScript Rabbit Anti-DDDDK-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
SpecificityThis Antibody recognizes the enterokinase cleavage site and specifically binds to fusion proteins containing C-terminal, N-terminal, and internal xxxDDDDK epitope tags, such as Xpress tag or Flag tag
Concentration1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
ImmunogenA synthetic epitope peptide xxxDDDDK conjugated to KLH. This sequence represents the enterokinase cleavage site (ECS).
Purification MethodImmunoaffinity chromatography
ApplicationsWorking concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using the ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) (GenScript, L00204C) is recommended. 5 µg of this antibody is mixed with 10 ml of WB solution for any 8 cm X 8 cm membrane.
Other applications: user-optimized
ConjugationUnconjugated
Host SpeciesRabbit
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
* For Non-Clinical Research Use Only *


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