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DDDDK-tag Antibody, pAb, Rabbit  |
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| Catalog No. |
Size |
Price |
Figures |
A00676-40 ug
| 40 ug | $ 50.00 | MSDS: 20081107004754 (PDF)
EXAMPLE:
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EXAMPLE:
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A00676-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | DDDDK-tag Antibody, pAb, Rabbit |
| | Alias | Rabbit Anti-DDDDK-tag pAb |
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| Abbreviated Name-2 | Anti-DDDDK-tag pAb |
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| Description | Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The xxxDDDDK-tag is a small size and high hydrophilicity epitope tag containing an enterokinase cleavage site that allows it to be completely removed from the fusion proteins.
The GenScript Rabbit Anti-DDDDK-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide containing xxxDDDDK epitope conjugated to KLH.
This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-DDDDK-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
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| Specificity | This Antibody recognizes the enterokinase cleavage site and specifically binds to fusion proteins containing C-terminal, N-terminal, and internal xxxDDDDK epitope tags, such as Xpress tag or Flag tag |
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| Concentration | 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide |
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| Immunogen | A synthetic epitope peptide xxxDDDDK conjugated to KLH. This sequence represents the enterokinase cleavage site (ECS). |
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| Purification Method | Immunoaffinity chromatography |
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| Applications | Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using the ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) (GenScript, L00204C) is recommended. 5 µg of this antibody is mixed with 10 ml of WB solution for any 8 cm X 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Host Species | Rabbit
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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| * For Non-Clinical Research Use Only *
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. | |
If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
