| Catalog No. |
Size |
Price |
Figures |
A00677-40 ug
| 40 ug | $ 50.00 | EXAMPLE:
Zoom
|
|---|
A00677-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | Rabbit Anti-tag•100-tag Polyclonal Antibody |
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| Alias | Rabbit Anti-tag•100-tag pAb |
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| Description | Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression in several different expression systems. Tag-100 tag is an epitope tag composed of a 12-residue peptide, EETARFQPGYRS, derived from the C-termini of mammalian MAPK/ERK kinases.
GenScript Rabbit Anti-tag epitope-100-tag polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 100 μl aliquot at a concentration of 1 mg/ml in PBS, pH7.4, containing 0.02% sodium azide. |
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| Ig Subclass | Rabbit IgG |
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| Storage | This product remains stable for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
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| Specificity | This antibody is specific to fusion proteins containing tag-100 epitope-tag. The antibody can also be used to detect human MAPK1 protein. |
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| Concentration | 1 mg/ml in PBS, pH7.4, containing 0.02% sodium azide |
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| Immunogen | Tag-100 epitope-tag peptide EETARFQPGYRS conjugated to KLH |
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| Purification Method | Immunoaffinity chromatography |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using One-Step WesternTM Kit: For quick results, One-Step WesternTM Complete Kit (GenScript, L00204C) is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Host Species | Rabbit |
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| Antigen Species | Human |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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