| Catalog No. |
Size |
Price |
Figures |
A00681-40 ug
| 40 ug | $ 50.00 | EXAMPLE:
Zoom
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A00681-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | Rabbit Anti-MAT-tag Polyclonal Antibody |
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| Alias | Rabbit Anti-MAT-tag pAb |
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| Abbreviated Name-2 | Anti-MAT-tag pAb |
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| Description | Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The MAT tag (Metal Affinity Tag) is a peptide tag that binds to transition metals such as nickel and cobalt. This tag allows the purification of MAT fusion proteins using immobilized metal affinity chromatography. GenScript Rabbit Anti-MAT-tag polyclonal antibody is developed in rabbit using a synthetic MAT tag peptide conjugated to KLH.
Rabbit Anti-MAT-tag polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml.
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-MAT-tag Polyclonal Antibody remains stable for 2-3 weeks at 4°C if stored. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript Rabbit Anti-MAT-tag polyclonal antibody specifically binds to over-expressed fusion proteins containing MAT epitope tag. |
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| Concentration | 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide |
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| Immunogen | Synthetic peptide conjugated to KLH |
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| Purification Method | Immunoaffinity chromatography |
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| Applications | Working concentrations for specific applications should be determined by the investigator. The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2.0 µg/ml
Western blot using the GenScript One-Step WesternTM Kit:
For quick results, we recommend the GenScript One-Step WesternTM Kit (L00204C). 15 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized
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| Conjugation | Unconjugated |
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| Host Species | Rabbit |
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| Note | Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap. |
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