| Catalog No. |
Size |
Price |
Figures |
A00682-40 ug
| 40 ug | $ 50.00 | MSDS: 20080228220610 (PDF)
MSDS: 20051008215330 (PDF)
EXAMPLE:
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EXAMPLE:
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A00682-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | Rabbit Anti-RFP-tag Polyclonal Antibody |
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| Alias | Rabbit Anti RFP-tag pAb |
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| Abbreviated Name-2 | Anti RFP-tag pAb |
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| Description | Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems. RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-RFP-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript Rabbit Anti-RFP-tag Polyclonal Antibody specifically reacts with fusion proteins containing RFP-tags. The antibody does not react with GFP or GFP-tagged proteins. |
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| Concentration | 1 mg/ml in PBS, pH7.4, containing 0.02% sodium azide |
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| Immunogen | RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated to KLH |
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| Purification Method | Immunoaffinity chromatography |
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| Applications | Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Host Species | Rabbit |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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