| Catalog No. |
Size |
Price |
Figures |
A00725-40 ug
| 40 ug | $ 50.00 | MSDS: 20080228041824 (PDF)
EXAMPLE:
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EXAMPLE:
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A00725-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | Rabbit Anti-SRT-tag Polyclonal Antibody |
|
| Alias | Anti-SRT-tag |
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| Description | Well-characterized antibodies for epitope tags consisting of short sequences are often used in the study of protein expression in various systems. The SRT-tag is a peptide epitope comprised of 10 amino acids derived from the crystalline surface layer protein of Rickettsia typhi1. GenScript Rabbit Anti-SRT-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic SRT tag peptide conjugated to KLH.
GenScript Rabbit Anti-SRT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-SRT-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript Rabbit Anti-SRT-tag is specific to fusion proteins containing SRT tags. |
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| Concentration | 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide |
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| Immunogen | Rabbit anti-SRT tag is a synthetic SRT tag peptide with the sequence TFIGAIATDT, conjugated to KLH |
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| Purification Method | Immunoaffinity chromatography |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using One-Step WesternTM Kit: For quick results, GenScript One-Step WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Host Species | Rabbit |
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| Antigen Species | Human |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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