| Catalog No. |
Size |
Price |
Figures |
A00730-40 ug
| 40 ug | $ 74.00 | EXAMPLE:
![Mouse Anti-β-Actin [HRP] Monoclonal Antibody,Western blot analysis](http://www.genscript.com/site2/document/3643_20071212210137.JPG)
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A00730-200 ug
| 200 ug | $ 333.00 |
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| Full Name | | THETM Anti-β-Actin [HRP] Monoclonal Antibody (Mouse) |
|
| Alias | mouse anti-β-actin [HRP] mAb |
|---|
| Description | THETM Anti-β-Actin [HRP] Monoclonal Antibody (Mouse) is immunoaffinity purified from mice ascites with protein G and then conjugated to horseradish peroxidase. GenScript THETM Anti-β-Actin [HRP] Monoclonal Antibody (Mouse) is supplied a 100 μg aliquot in the lyophilized form. Do not add azide; it inhibits HRP activity. |
|---|
| Ig Subclass | Mouse IgG2a |
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| Storage | GenScript THETM Anti-β-Actin [HRP] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored between 2°C and 8°C. The reconstituted antibody can be stored for up to three months between 2°C and 8°C or for up to two years at or below -20°C. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript THETM Anti-β-Actin [HRP] Monoclonal Antibody (Mouse) reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig substrates. It has not yet been tested in other species.
|
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| Concentration | 40 μg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal. |
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| Immunogen | peptide (DDDIAALVVDNGSG) conjugated to KLH |
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| Purification Method | Protein G affinity column |
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| Reconstitution | Reconstitute GenScript THETM Anti-β-Actin [HRP] Monoclonal Antibody (Mouse) (40 μg) with 100 μl of deionized water to a final antibody concentration of 0.4 mg/ml. |
|---|
| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 1.0 µg/ml
Other applications: user-optimized |
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| Fusion Partner | Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells. |
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| Conjugation | Horseradish peroxidase (HRP) |
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| Host Species | Mouse |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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