| Catalog No. |
Size |
Price |
Figures |
A00864-40 ug
| 40 ug | $ 74.00 | EXAMPLE:
![Mouse Anti-c-Myc Monoclonal Antibody[Biotin]](http://www.genscript.com/site2/document/3644_20071212211551.JPG "Mouse Anti-c-Myc Monoclonal Antibody[Biotin]")
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A00864-200 ug
| 200 ug | $ 333.00 |
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| Full Name | | THETM Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) |
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| Alias | Anti-c-Myc [Biotin] |
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| Description | GenScript THETM Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) is highly immunoaffinity purified from mice ascites by protein A and then conjugated to biotin. GenScript Anti-c-Myc [Biotin] Monoclonal Antibody is suitable for detecting the expression level of c-Myc in fusion proteins. |
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| Ig Subclass | Mouse IgG2a |
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| Storage | GenScript THETM Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored 2-8°C. The reconstituted antibody can be stored for up to three months 2-8°C or for up to two years at or below -20°C. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript THETM Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc gene in human cancers, such as Hela whole cell lysates. |
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| Concentration | 40 μg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide and 100 μg, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
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| Clone | A00864.01 |
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| Immunogen | A synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH |
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| Purification Method | Protein A affinity column |
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| Reconstitution | Reconstitute lyophilized GenScript THETM Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) (40 μg or 100 μg) with 100 μl of deionized water to a final antibody concentration of 0.4 mg/ml or 1 mg/ml. |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Other applications: user-optimized |
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| Fusion Partner | Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells. |
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| Conjugation | Biotin |
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| Host Species | Mouse |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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