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THETM Anti-GST Monoclonal Antibody (Mouse)

Catalog No. Size Price Figures
A00865-40 ug
 		40 ug
$ 69.00
MSDS:  20080228042104  (PDF)

EXAMPLE:
Mouse Anti-GST mAb
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EXAMPLE:
western analysis using Mouse Anti-GST mAb
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EXAMPLE:
TheTM Anti-GST Monoclonal Antibody (Mouse) one-step WB
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A00865-200 ug
 		200 ug
$ 311.00
A00865-500 ug
 		500 ug
$ 750.00
Full Name
THETM Anti-GST Monoclonal Antibody (Mouse)
AliasMouse Anti-GST monoclonal antibody
DescriptionSchistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.
Key Features
  • THETM Anti-GST Monoclonal Antibody (Mouse) has high sensitivity, better than the antibody in the market.
  • GenScript THETM Anti-GST Monoclonal Antibody (Mouse) can recognize N-terminal, C-terminal and internal GST tag in fusion proteins.
    • Ig SubclassIgG1, k
    StorageTHETM Anti-GST Monoclonal Antibody (Mouse) should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.
    SpecificityTHETM Anti-GST Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
    Concentration40 µg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide and 100 µg, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
    Note: 500 µg (GenScript, A00865), no sodium azide included
    Clone2F10B9
    ImmunogenRecombinant GST protein (26 kD)
    Purification MethodProtein A affinity column
    ReconstitutionReconstitute the lyophilized antibody (40 µg, 100 µg, 200 µg, or 500 µg) with 80 µl, 100 µl, 400 µl, or 1000 µl of deionized water (or equivalent) to the final concentration of 0.5 mg/ml.
    ApplicationsWorking concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.2 µg/ml
    Western blot: 0.1-1 µg/ml
    Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2 ml of PBST).
    Immunoprecipitation (IP): 1 µg/ml
    Other applications: user-optimized
    Fusion PartnerSpleen cells were fused with SP2/0-Ag14 mouse myeloma cells
    ConjugationUnconjugated
    Host SpeciesMouse
    NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
    * For Non-Clinical Research Use Only *
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    A00866antibody : THETM Anti-GST [HRP] Monoclonal Antibody (Mouse)40 ug
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