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THETM GST Antibody, mAb, Mouse

Catalog No. Size Price Figures
A00865-40 ug
 		40 ug
$ 69.00
MSDS:  20080118034804  (PDF)
MSDS:  20080228042104  (PDF)
COA:  A00865_10K000193  (PDF)
COA:  A00865_10K000213  (PDF)
COA:  A00865_11I000353  (PDF)

EXAMPLE:
Mouse Anti-GST mAb
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EXAMPLE:
western analysis using Mouse Anti-GST mAb
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EXAMPLE:
TheTM Anti-GST Monoclonal Antibody (Mouse) one-step WB
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A00865-100 ug
 		100 ug
$ 175.00
A00865-200 ug
 		200 ug
$ 311.00
A00865-500 ug
 		500 ug
$ 750.00
Full Name
THETM GST Antibody, mAb, Mouse
AliasMouse Anti-GST monoclonal antibody
DescriptionSchistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.
Key Features
  • THETM GST Antibody, mAb, Mouse has high sensitivity, better than the antibody in the market.
  • GenScript THETM Anti-GST Monoclonal Antibody (Mouse) can recognize N-terminal, C-terminal and internal GST tag in fusion proteins.
    • Ig SubclassIgG1, k
    StorageThe antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
    SpecificityTHETM Anti-GST Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
    Concentration40 µg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
    Note:
    1. 100 µg(GenScript, A00865), lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
    2. 500 µg (GenScript, A00865), lyophilized, without sodium azide or carrier protein
    Clone2F10B9
    ImmunogenRecombinant GST protein (26 kD)
    Purification MethodProtein A affinity column
    ReconstitutionReconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
    ApplicationsWorking concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.2 µg/ml
    Western blot: 0.1-1 µg/ml
    Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2 ml of PBST).
    Immunoprecipitation (IP): 1 µg/ml
    Other applications: user-optimized
    Fusion PartnerSpleen cells were fused with SP2/0-Ag14 mouse myeloma cells
    ConjugationUnconjugated
    Host SpeciesMouse
    NoteSmall volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap.
    Technical ManualTM0589, TM0590, TM0591, TM0592, TM0593
    * For Non-Clinical Research Use Only *
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